Correlation Between Cow’s Milk Protein Allergy And Vitamin D Level In Infants And Analysis Of The Effect Of The Combined Action Of Bifidobacteria

Objective:To investigate whether there is a correlation between CMPA and serum vitamin D levels,and the changes of serum vitamin D level and digestive tract allergy symptoms of CMPA after combined supplementation of vitamin AD and bifidobacterium.To explore the adjuvant clinical therapeutic value of vitamin D and bifidobacteria on CMPA.Methods:Vitamin AD levels and related clinical data were collected from children with milk protein allergy and healthy children of the same age,who had attended the paediatric department of the Affiliated Hospital of Chuanbei Medical College from January 2021 to January 2022.According to inclusion criteria,healthy children were selected as the healthy group and children with milk protein allergy were selected as the allergic group.After being diagnosed with milk protein allergy,children with digestive tract allergy symptoms were selected and fed with deep hydrolyzed protein milk powder.At the same time,they were randomly divided into a control group and an experimental group.The control group was given the usual oral supplementation of vitamin AD,vitamin A 1500 IU and vitamin D 900IU daily for 3 months.The experimental group was given the same amount of vitamin AD drops and bifidobacterium tablets,3 times a day,0.5g once,7 days one month,for 3 months.After 3 months,changes in vitamin D levels and improvement in gastrointestinal allergy symptoms were observed in both groups.Result:1.In this study,serum vitamin D levels were analysed in 86 healthy children and 71 children with milk protein allergy,and we found that vitamin D levels in the allergic group were(35.09±10.85 ng·mL-1)and in the healthy group were(39.50(33.60,43.95)ng·mL-1).Vitamin D levels were significantly lower in the allergic group than in the healthy group,with a statistically significant difference(P<0.05).2.The analysis of clinical symptoms of allergic group found that the most significant allergic symptoms were in the digestive system,about 70.42%of the allergic group had diarrhea and bloody stool symptoms,followed by atopic dermatitis,and respiratory symptoms were rare.At the same time,it was found that the allergic symptoms mainly involving two systems,accounting for 52.11%of the allergic group,mainly manifested as digestive tract and skin allergy symptoms.However,serum vitamin D levels were not significantly different from each other,whether one or more systems were involved(P>0.05).There was no significant correlation between vitamin D levels and severity of clinical symptoms.3.The eosinophil count,eosinophil percentage and platelet count of peripheral blood in the allergic group were significantly higher than those in the healthy group.The difference between the two groups was statistically significant(P<0.05).No statistically significant difference between the two groups in terms of white blood cell count(P>0.05).4.Vitamin D levels in the experimental group and control group were both significantly increased after supplementation,with statistical significance between the two groups(P<0.05).Vitamin D level(5.98±0.79)ng·mL-1 in the experimental group and(4.39±0.85)ng·mL-1 in the control group after supplementation.The difference in vitamin D levels between the two groups was statistically significant(P<0.05),with the experimental group showing a more significant increase in vitamin D levels than the control group.5.We compared the gastrointestinal allergy symptom relief time of the experimental group with control group,it was shorter in the experimental group(7.12±1.37 d)than in the control group(8.36±1.41 d),statistically different between the two groups(P<0.05).Conclusion:1.Low vitamin D levels are associated with CMPA,but vitamin D level cannot be used to assess the severity of clinical symptoms in children with CMPA.2.The changes of eosinophil and platelet in peripheral blood have certain evaluation value for CMPA.3.Simultaneous supplementation of vitamin D and bifidobacteria for children with CMPA can further promote vitamin D absorption and accelerate the improvement of digestive tract allergy symptoms,which is expected to be a new adjuvant treatment for CMPA in the future.