| Objective: This paper studies and describes the effects of isoproterenol on renal pelvis pressure and rabbit vital signs after perfusion of rabbit kidneys with different concentrations of isoproterenol,so as to obtain the optimal perfusion concentration.Methods: In this experiment,40 white rabbits were randomly divided into four groups,A,B,C,and D.The F3 epidural puncture catheter was placed in the left renal pelvis and connected to the perfusion catheter and pressure measurement system.The renal pelvis was perfused with 1.0ug/ml,0.9ug/ml,0.8ug/ml isoproterenol solution and normal saline,and then the femoral artery was punctured and connected to the manometry system.Monitor renal pelvis pressure,mean arterial pressure,heart rate and blood drug concentration at5min(-5min),start of perfusion(0min),4min,8min,16 min and 25 min before perfusion.The data obtained were analyzed by statistical methods for their expression.Results: A total of 40 rabbit specimens were collected,of which 3died due to excessive anesthesia.The final data analysis of 37 rabbit specimens showed that the average weight of the rabbits was 2.98±0.05 kg,the average age was 148.12±2.42 days.(1)Renal pelvis pressure: The renal pelvis pressure of all experimental rabbits increased suddenly after the start of perfusion.The pressure measured in group A was 81.44±12.98 mm Hg at 4 minutes,and decreased by 11.6%,49.4% and 73.7% at 8,16,and 25 minutes,respectively(P<0.01).The pressure measured in group B was 83.40±7.55 mm Hg at 4 minutes,and decreased by 12.8%,33.1% and 49.8% at 8,16,and 25 minutes,respectively(P<0.01).The pressure measured in group C at 4 minutes was 81.35±9.07 mm Hg,decreased by 12.9%,33.7% and 49.6% at 8,16,and 25 minutes,respectively(P<0.01).The renal pelvis pressure in group D did not decrease after the increase(P>0.01).(2)Mean arterial pressure: The mean arterial pressure measured at the beginning of perfusion(0min)in group A was53.48±5.45 mm Hg,and the mean arterial pressure at 4,8,16,and 25 minutes increased by 2.8%,5.6%,10.1% and 17.3%(P<0.01),and there was no significant change in the whole process of group B,C,and D(P>0.01).(3)Average heart rate: The average heart rate measured at the start of perfusion(0min)in group A was 268.02±11.12 beats/min,and the average heart rate at 4,8,16,and 25 minutes increased by 0.43%,1.02%,3.35% and 6.18%,respectively(P<0.01).There was no significant change in group B,C,D in the whole process(P>0.01).(4)Blood concentration:A very low dose of isoproterenol could be detected in the blood of all experimental groups after perfusion.The blood concentration of group A was 44.41±0.47pg/ml at 4min,and the blood concentration of group A increased 141%,288% and 335% at 8,16 and 25 min,respectively(P<0.01).The blood concentration of group B was 31.12±0.83pg/ml at 4min,and increased 145%,195% and 213% at 8,16 and 25 min,respectively(P<0.01).The blood concentration of group C was29.18±0.52pg/ml at 4min.At 8,16 and 25 min,the serum concentrations increased by 126%,176% and 237%,respectively(P<0.01).No isoproterenol was detected in group D(P >0.01).Conclusion: 1.The renal pelvis pressure can be reduced when a certain concentration of isoproterenol is used as the perfusion solution to the rabbit kidney.2.When the concentration of isoproterenol is 1ug/ml,the renal pelvis pressure is reduced more obviously,but it also affects the blood pressure and heart rate of rabbits,so the safe concentration is below 1ug/ml.3.Although all the above results were obtained from animal experiments,they are potentially relevant to clinical practice and provide a theoretical basis for clinical measures to effectively reduce renal pelvis pressure. |
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