Comparative Analyses On The Active Constituents And Pharmacological Activities Of Discarded Branches,Leaves And Stems From Nauclea Officinalis Of Li Nationality Medicine

Objective: Enterprises that use Nauclea officinalis of Li Nationality medicine as raw material to produce medicines such as Dan Mu Jin Gao Tang Jiang,discard the branches and leaves during the harvesting and processing of the stems from Nauclea officinalis,which not only increases the cost of Nauclea officinalis processing,but also causes waste of Nauclea officinalis resources.In order to fully utilize the waste resources of Nauclea officinalis,and to deeply explore the potential value of the waste branches and leaves as supplementary resources to the stem of medicinal parts,it is necessary to conduct an in-depth comparative study on the active components and pharmacological activities of the branches,leaves and stems.Methods: Applying databases and software such as Swiss ADME,Swiss Target Prediction and DAVID Bioinformatics Resources,the active constituents from Nauclea officinalis of Li Nationality medicine were determined by the network pharmacology;Liquid Chromatography couple Tandem Mass Spectrum was used to determine the content of bioactive components in different parts of Nauclea officinalis;The antipyretic,analgesic,anti-inflammatory and antibacterial activities of different parts from Nauclea officinalis were compared through using the rabbit fever model induced by endotoxin,the mice pain model induced by acetic acid,the mice capillary permeability increase model induced by acetic acid,the mice ear swelling model induced by xylene,and measuring the diameter of bacteriostatic.Results:1.Study on bioactive components of Nauclea officinalis It was found that strictosamide、vincosamide、naucleactonin A、naucleactonin B、naucleamide B、naucleamide G、pumiloside、sweroside、protocatechuic acid、chlorogenic acid、cryptochlorogenic acid and vanillic acid were the main bioactive components in Nauclea officinalis.These bioactive components can regulate NF-κB VIsignal pathway and inflammatory mediator of tryptophan channels by acting on extracellular regions,plasma membrane and other sites,binding with related proteins and participating in biological processes such as nitric oxide biosynthetic process and cell adhesion of leukocyte,which makes Nauclea officinalis exert its antipyretic,analgesic and anti-inflammatory effects.2.Comparative analysis of the content of bioactive components in waste branches,leaves and stems of Nauclea officinalisThe active ingredients,strictosamide,vincosamide,naucleamide B,pumiloside,sweroside,protocatechuic acid,chlorogenic acid,cryptochlorogenic acid and vanillic acid,which were isolated and collected,were used as the index components to determine the content of different parts in Nauclea officinalis.The study found that the branches,leaves and stems of Nauclea officinalis all contain alkaloid active components,and the naucleamide B content in the branches and leaves is higher than that in the stems(the branches,leaves and stems are 0.33±0.034 μg/g,0.423±0.064μg/g,and 0.183±0.055 μg/g respectively).The content of pumiloside is in the order of branch>stem>leaf.Although the content of strictosamide and vincosamide is lower than that of the stem from Nauclea officinalis,the branches and leaves still contain a certain amount;There was no significant difference in the content of chlorogenic acid in different parts of Nauclea officinalis,but there were not detected protocatechuic acid and vanillic acid in different parts of Nauclea officinalis;The content of cryptochlorogenic acid in the leaves of Nauclea officinalis was the highest(5780.07±1785.16 μg/g),and there was no significant difference in this active component between branches and stems;There was no significant difference in the content of sweroside in the branches compared with those in the stems of Nauclea officinalis,while this active component was not detected in the discarded leaves of Nauclea officinalis.3.Comparative analysis of pharmacological activities in waste branches,leaves and stems of Nauclea officinalis The branches and leaves of Nauclea officinalis can reduce the temperature rise rate in rabbits,the permeability of capillaries in mice,and inhibit the ear swelling of mice,compared with the stems of the medicinal parts,the branches and leaves of Nauclea officinalis showed stronger antipyretic and anti-inflammatory activities;The different parts of Nauclea officinalis could significantly reduce the number of writhing in mice(P<0.001),and had a significant inhibitory effect on Escherichia coli and Staphylococcus aureus(P<0.01),different parts from Nauclea officinalis showed strong analgesic and antibacterial activity.Conclusions: The bioactive components of Nauclea officinalis mainly include strictosamide、vincosamide、naucleactonin A、naucleactonin B、naucleamide B、naucleamide G、pumiloside、sweroside、protocatechuic acid、chlorogenic acid、cryptochlorogenic acid and vanillic acid;The bioactive components of the abandoned parts from Nauclea officinalis are generally the same as those of the medicinal parts,and even some bioactive components(Pumiloside 、 Naucleamide B 、Cryptochlorogenic acid)are higher than the stems;The analgesic and antibacterial pharmacological activities of different parts from Nauclea officinalis of Li nationality medicine have similar effect intensity,but the antipyretic and anti-inflammatory activities of branches and leaves are stronger.The above researches provide a scientific basis for the reuse of the abandoned branches and leaves of Nauclea officinalis,which is conducive to reducing the production cost of enterprises and the waste of Nauclea officinalis resources,and is conducive to improving economic and social benefits for the benefit of mankind.