| Objective: To observe the effects of Shufeng Xuanfei Decoction and its combination with Montelukast on IL-33,IL-10,airway inflammation and airway remodeling in CVA model rats.Methods: The rat model of CVA was established by means of ovalbumin(OVA)combined with alumina hydroxide gel intraperitoneal injection sensitization and OVA atomization stimulation.25 SD rats were randomly divided into the following five groups: normal group,model group(CVA group),Western medicine group(Montelukast treatment group),Traditional Chinese medicine group(Shufeng Xuanfei decoction treatment group),combined group(Montelukast combined Shufeng Xuanfei Decoction treatment group),5 rats in each group.Day 8,the model group,Western medicine group,Chinese medicine group and combined group were intraperitoneally injected with 2 mg ovalbumin(OVA)and 100 mg aluminum hydroxide gel suspension sensitization solution0.2 ml.Day 29,the same dose of suspension sensitization solution was repeated once,and the normal group was intraperitoneally injected with 0.2 ml normal saline.Day 50,except normal group,all groups were atomized with 1% OVA for20 minutes,once every other day,7 times in total.Each group was given drug intervention 30 minutes before nebulization excitation for 14 days.The drug was converted according to the proportion of human(70 kg)and rat body surface area.The Chinese medicine group was given Shufeng Xuanfei Decoction 8.55g/kg intragastric administration,2 ml/day.The Western medicine group was given Montelukast 0.9 mg/kg intragastric administration,2 ml/day.The combined group was given Shufeng Xuanfei Decoction 8.55 g/kg and Montelukast 0.9 mg/kg,each 2 ml/day.The normal group was given normal saline intragastric administration,2 ml/day.The general condition of rats was observed in sensitization and stimulation stages,and the cough sensitivity and special airway resistance were measured 24 hours after the last stimulation.Bronchoalveolar lavage fluid(BALF)and lung tissue were collected from each group of rats,and the contents of IL-33 and IL-10 in BALF were detected by ELISA.The pathological changes of lung tissue in each group of rats were observed by H&E staining,PAS staining and MASSON staining method.The expression of transcription factor IL-33 and Fox P3 protein in lung tissues was detected by immunohistochemistry.Results: 1.General observation of rats in each group: Compared with the model group,rats in each treatment group showed less frequently scratching their face and nose,sneezing,coughing,agitation,incontinence in the early stage of excitation,and improved burnout,reduced activity,rough hair and so on performance in the late and the normal group did not have the above performance.2.Cough frequency comparison results: Compared with the normal group,the number of coughs in the model group was significantly increased(P<0.05).Compared with the model group,the number of coughs in all treatment groups were decreased in different degrees(P<0.05).The number of coughs in the combined group was decreased most significantly,and it was significant that compared with the Western medicine group and the Chinese medicine group(P<0.05).but there was no significant difference between the western medicine group and the Chinese medicine group(P>0.05).3.Special airway resistance(s Raw)comparison results: Compared with the normal group,the airway resistance of the model group increased(P<0.05).Compared with the model group,the airway resistance decreased in each treatment group(P<0.05),and the airway resistance of the combined group was more obviously decreased than that in the Chinese medicine group and the Western medicine group(P<0.05).but there was no significant difference between the Chinese medicine group and the Western medicine group(P>0.05).4.ELISA detection showing:Compared with the normal group,the IL-33 content in the model group BALF was significantly increased(P<0.05)and the IL-10 content was reduced(P<0.05).Compared with the model group,the IL-33 content in BALF in all treatment groups decreased and the IL-10 content increased(P<0.05).The combined group had the lowest IL-33 content and the highest IL-10 content,which was significantly different with the Chinese medicine group and the Western medicine group(P<0.05).There was no significant difference in the contents of 1L-33 and IL-10 between the Chinese medicine group and the Western medicine group(P>0.05).5.Observation of pathological results of lung tissue: The lung tissue of rats in the model group showed significant thickening of airway smooth muscle,basement membrane hyperplasia,lumen stenosis.A large number of viscous secretions,exfoliated epithelial cells and inflammatory cells can be seen in the lumen of the tube.Inflammatory cell infiltration can also be seen in the periphery of the bronchi,pul-monary interstitium,and alveolar lumen.Goblet cell hyperplasia can also be seen in the airway mucosa,and obvious deposition of collagen fibers under the epithelium of the airway can also be seen in the lung tissue of CVA rats.The above changes were improved in all treatment groups,especially in the combined group.6.Immunohistochemical detection of IL-33 and Fox P3 protein expression results in lung tissues:Compared with the normal group,the IL-33 expression of pulmonary tissue in the model group rats was increased(P<0.05),and foxp3 expression was reduced(P<0.05).Compared with the model group,the IL-33 expression of pulmonary tissue was reduce(P<0.05)and the Fox P3 expression of pulmonary tissue increased(P<0.05)in all treatment groups,and the combination group had the most significant change(P<0.05).Conclusions: 1.Shufeng Xuanfei Decoction can improve cough sensitive,airway hyperreactivity,airway inflammation and airway remodeling in CVA rats,reducing the number of goblet cells in airway mucosa and the deposition of subepithelial collagen fiber,and improving myofibroblast hyperplasia.2.Shufeng Xuanfei Decoction can reduce the content of pro-inflammatory factor IL-33 in BALF of CVA rats,and increase the level of anti-inflammatory factor IL-10,restoring the imbalance between pro-inflammatory and anti-inflammatory cytokines.3.Shufeng Xuanfei Decoction can reduce the expression of IL-33 and increase the expression of Fox P3 in lung tissues of CVA rats.These results suggest that it may play a therapeutic role by interfering with upstream pathways of related cytokines.4.The combined treatment of Shufeng Xuanfei Decoction and Montelukast was superior to monotherapy,and the intervention effect on CVA rats was more significant than monotherapy. |
PDF Full Text Request