Screening Of Cardiac Hypertrophy-associated MicroRNAs And Analysis Of Their Mechanisms Of Action Based On GEO Database

Objective:Based on the National Center For Biotechnology Information(NCBI)gene expression omnibus(GEO),we used bioinformatics analysis to screen for micro RNAs associated with human cardiac hypertrophy and analyze their mechanisms of action.Methods:The human cardiac hypertrophy datasets GSE60292 and GSE60291 were obtained from the GEO database,the differentially expressed miRNAs and differentially expressed messenger RNAs(mRNAs)in the datasets were screened using the limma package,the known target genes of the differentially expressed miRNAs were retrieved in the miRbase database,the retrieved genes and the differentially and the differentially expressed mRNAs in the GSE60291 dataset were intersected,and the mRNAs in the intersection were enriched for gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways using the DAVID database to analyze the role of miRNAs and their molecular mechanism.Results:A total of 243 differentially expressed miRNAs and 488 differentially expressed mRNAs were screened in the cardiac hypertrophy dataset with the limma package,and there were 9886 target mRNAs predicted to differentially express miRNAs in the miRbase database,and 488 mRNAs in both intersection sets.GO analysis of biological process(BP)gene enrichment was focused on regulation of protein serine,epithelial cell proliferation,regulation of MAP kinase activity,muscle organ development regulation,etc.Cellular component(CC)enrichment was concentrated in microtubule,cell-substrate junction,spindle,myofibril,etc.which indicates that some of our screened mRNAs are involved in microtubule formation as well as functional regulation.Molecular function(MF)enrichment was focused on signaling receptor activator activity,microtubule binding,protein kinase regulator activity,etc.KEGG pathway enrichment analysis focused on MAPK signaling pathway(MAPK),P53 signaling pathway,Homologous recombination,DNA replication,etc.Conclusion:Through the analysis of the GEO database myocardial hypertrophy dataset,miR-208 a,miR-340-5p and other micro RNAs appeared to be differentially expressed during myocardial hypertrophy,and KEGG enrichment analysis revealed that MAPK signaling pathway may be an important pathway in the development of myocardial hypertrophy,and miRNAs may promote or inhibit myocardial hypertrophy by regulating MAPK pathway.This paper provides ideas for the study of the mechanism of miRNA regulation of myocardial hypertrophy and suggests signaling pathways that can be further investigated.