Construction Of Competitive Endogenous RNA Network In Peripheral Blood Monocytes Of Patients With Coronary Artery Disease

Objective:To construct and analyze the competitive endogenous RNA network composed of circ RNAs in peripheral blood monocytes of patients with coronary artery disease.Methods:Differentially expressed circ RNA,mi RNA and m RNA were identified from GEO dataset GSE115733,GSE105449 and GSE166780,respectively.The circ RNA-mi RNA interaction and mi RNA-m RNA interaction were predicted by mi Randa software and Targetscan software respectively.According to ce RNA hypothesis,the triple relationship of m RNA-mi RNA-down-circ RNA-up and m RNA-down-mi RNA-up-circ RNA-down was selected and the network diagram was drawn using Cytoscape software.GO function analysis and KEGG pathway analysis were performed with the m RNA in the interaction network.Finally,protein-protein interaction analysis was performed by using STRING database and Cytoscape software to find hub genes.Results:According to p-value < 0.05,13591 differentially expressed circrnas were screened from GSE115733,among which 6011 were highly expressed and 7580 were low expressed.A total of 12,888 different circ RNA-mi RNA-m RNA interactions were predicted,including 61 circ RNAs,25 mi RNAs and 872 m RNAs.The top six differentially expressed circ RNAs,their corresponding 11 mi RNAs and 95 m RNAs with|log FC≥5| were chosen to draw a network map.The complete protein-protein interaction network constructed from m RNA in ce RNA network contained 449 gene nodes and 736 interaction relationships.Finally,the selected hub genes were TNF,CCL20,CXCL2,CCL2,IL1 A,CCL5,CSF3,CCL3L3,MAPK3,MAPK1,RAC1 and MAP2K1.Conclusion:In this study,novel circ RNA-mi RNA-m RNA interaction network in peripheral blood monocytes of patients with coronary artery disease was constructed.In this network,there were 61 circ RNAs including hsa＿circ＿0069996,hsa＿circ＿0053283,hsa＿circ＿0067734,etc,25 mi RNAs including hsa-mir-142-3p,hsa-mir-376c-5p,hsa-mir-376a-3p,etc,and 872 m RNAs including CSF3,TNF,CCL20,etc.Among these interactions,the hsa＿circ＿0069094/hsa-mi R-200a-3p/CSF3 axis may play an important role in the process of monocyte mediated atherosclerosis.