Effects And Mechanism Of CD95 On Caski Cells Of Cervical Cancer

PurposeThe study aimed to investigate the effects of CD95 on proliferation,invasion and apoptosis of cervical cancer Caski cells,and explore its possible mechanism of function.This study was designed to provide a new therapy target for cervical cancer.MethodsCaski cells were selected as experimental subjects,and they were treated with CD95 antibodies at 0μg/m L,0.313μg/m L,0.625μg/m L,1.25μg/m L,2.5μg/m L,5μg/m L for 24 h and 48 h,the cell viability was detected by Cell Counting Kit-8(CCK8).Caski cells were treated with CD95 antibodies at 0μg/m L,1.25μg/m L,5μg/m L for 48 h,and cell proliferation was detected by CCK8;Cell Cycle and apoptosis were detected by flow cytometry(FCM).Transwell assay was used to detect cell invasion.Immunofluorescence(IF)and Western blot(WB)were used to detect the expression of downstream apoptotic proteins caspase-3,caspase-8,caspase-9 and p38 in the mitogen-activated protein kinase(MAPK)pathway after CD95 antibodies treatment.ResultsCD95 antibodies could inhibit the proliferation of Caski cells and promote the apoptosis of Caski cells.CD95 antibodies at 1.25μg/m L had no effect on the cell cycle progression of Ca Ski cells,but CD95 antibodies at 5μg/m L caused Caski cells to arrest in G0/G1 phase.CD95 antibodies inhibited cell invasion.After Caski cells were treated with CD95 antibodies,the proteins expressions levels of Caspase 3,Caspase 8,Caspase 9 and p38 were increased.ConclusionThe results showed that the CD95 antibodies could inhibit the proliferation and invasion of Caski cells,and promote the apoptosis.The effects of CD95 antibodies on Caski cells of cervical cancer may be related to the regulation of p38 protein in the MAPK pathway.