A Preliminary Study On The Association Between CTR Gene Polymorphism And Severe Periodontitis

Objective:The Sanger sequencing technology was used to determine whether there were differences in the distribution of calcitonin receptor(CTR)genotype and allele frequency between patients with severe periodontitis and healthy people,and to explore the association between CTR gene polymorphism and severe periodontitis.Methods:According to the inclusion criteria,114 patients with severe periodontitis and132 periodontal healthy subjects were included and classified as P group and H group,respectively.These two groups were further divided into four subgroups according to gender:the female periodontitis group(XP group),the female health group(XH group),the male periodontitis group(YP group)and the male health group(YH group).Peripheral anticoagulant blood was collected for DNA extraction,and PCR was performed for CTR rs1801197 and rs1042138.The amplified products were sequenced by the ABI 3730XL genetic analyzer.Genotypes and allele frequencies of CTR rs1801197 and rs1042138 were determined.Statistical analysis was conducted using the SPSS 26.0 software.The main statistical methods were Chi-square test and binary logistic regression analysis.Results:1.Three genotypes(GG,AG and AA)were detected at rs1801197 and rs1042138of CTR gene in our study population.The genotypes(GG,AG and AA)of rs1801197 were66.7%,31.6%and 1.8%in P group,and 71.2%,26.5%and 2.3%in H group,respectively.The genotypes(GG,AG and AA)of rs1042138 were 37.7%,41.2%and 21.1%in P group,and 34.1%,58.3%and 7.6%in H group,respectively.There was no significant difference in the genotype distribution and the allele frequencies of rs1801197 between P group and H group(P＞0.05).The genotype distribution of rs1042138 was significantly different between P group and H group(χ~2=11.814,P=0.003).The distribution of AA genotype in P group(21.1%)was significantly higher than that in H group(7.6%).There was no significant difference in the allele frequencies of rs1042138 between P group and group H group(P＞0.05).2.In the female study population,there was no significant difference in the genotype distribution and the allele frequencies of rs1801197 between XP group and XH group(P＞0.05).The genotype distribution of rs1042138 was significantly different between XP group and XH group(χ~2=10.239,P=0.006).The distribution of AA genotype in XP group(22.7%)was significantly higher than that in XH group(4.0%).There was no significant difference in the allele frequencies of rs1042138 between XP group and XH group(P＞0.05).3.In the male study population,there was no significant difference in the genotype distribution and the allele frequencies of rs1801197 between YP group and YH group(P＞0.05).There was no significant difference in the genotype distribution and the allele frequencies of rs1042138 between YP group and YH group(P＞0.05).4.Age,gender,smoking and two locus genotypes were included in the binary logistic regression model,and the results showed that severe periodontitis was correlated with age and rs1042138 genotype(P＜0.05),but not with gender,smoking and rs1801197 genotype(P＞0.05)under the recessive model of inheritance.Under the dominant model of inheritance,severe periodontitis was correlated with age and smoking(P＜0.05),but not with gender,rs1801197 genotype and rs1042138 genotype(P＞0.05).Conclusion:Under the present experimental:1.There was no significant correlation between CTR rs1801197 gene polymorphism and severe periodontitis.2.CTR rs1042138 gene polymorphism may be associated with severe periodontitis,and the effect of rs1042138 on severe periodontitis has gender difference,and female AA genotype carriers are susceptible to severe periodontitis.