Protective Effect Of Mongolian Medicine Deduhonghua-7 Powder On Chronic Liver Injury In Rats And Effects Of Iron Death Related Protein GPX4,DMT1,FTMTmRNA Expression

Objective:To observe the therapeutic effect of Mongolian medicine Dedu honghua-7 Powder on chronic liver injury induced by CCI₄and the effects of iron accumulation and mRNA expression of glutathione peroxidase 4（GPX4）,divalent metal transporter 1（DMT1）and mitochondrial ferritin（FTMT）in liver tissue.Methods:40 male SD rats were randomly divided into blank group,model group,silybin group and deduhonghua-7 powder group according to body weight,with 10 rats in each group.Except blank group,liver fibrosis model was established by CCl₄intritoneal injection.Blank group and model group were intragastrically fed with distilled water,Silybin group was intragastrically fed with silybin 0.021 g·kg^-1,and deduhonghua-7 powder group was intragastrically fed with deduhonghua-7 powder 0.3g·kg^-1for 7 weeks.After 7 weeks,liver tissue was collected,and fibrosis and inflammatory activity were evaluated by HE staining,collagen area was evaluated by masson staining,iron accumulation in liver tissue was observed by Prussian blue staining,and mRNA expressions of GPX4,DMT1 and FTMT in liver tissue of rats in each group were measured by reverse transcription polymerase chain reaction（RT-PCR）.Results:1.HE staining results Blank group:liver lobules were clearly demarcated,liver cells centered on the central vein were arranged radially,and no changes of fibrous proliferation or inflammatory cell infiltration were observed.Model group:lobular structure disorder,hepatic cell steatosis,inflammatory cell infiltration in part of the portal area,interlacing of hyperplastic fibers to form fibrous septa;Silybin group and deduhonghua-7powde group:the granular degeneration,lobular structure disorder and fibrous hyperplasia of liver cells were lighter than those of model group.2.Masson staining results Blank group:no obvious hyperplasia of collagen fibers;Model group:collagen fibers were hyperplasia around the central vein and portal area,and lobules were disorganized.A small amount of blue dye collagen was found in silybin group and deduhonghua-7 powde group.The percentage of collagen area to total area of liver tissue in each group was:blank group（0.80±0.29）%,model group（14.84±4.45）%,silybin group（8.82±2.63）%,deduhonghua-7 powde group（7.99±1.71）%,respectively.Compared with blank group,the percentage of collagen area in model group,silybin group and deduhonghua-7powde group was increased,and there was significant difference（P<0.05）;Compared with model group,the percentage of collagen area in silybin group and deduhonghua-7 powde group decreased,and there was a significant difference（P<0.05）.3.Prussian staining results blank group:No Prussian blue staining area;Model group:multiple spotty or patchy blue staining areas;A few blue staining spots were observed in silybin group and deduhonghua-7 powde group.The percentage of Prussian blue staining area in liver tissue of rats in each group was blank group（0.00±0.00）%,model group（1.37±0.48）%,silybin group（0.31±0.11）%,deduhonghua-7 powde group（0.68±0.16）%,respectively.Compared with blank group,the percentage of Prussian blue staining area in model group,silybin group and deduhonghua-7 powde group was increased,and there was significant difference（P<0.05）;Compared with model group,the percentage of Prussian blue dye area in silybin group and deduhonghua-7powde group was decreased,and there was a significant difference（P<0.05）.4.The mRNA expressions of GPX4,DMT1 and FTMT in rat liver tissue were detected by RT-PCR Blank group（2.09±0.57）,model group（0.99±0.16）,silybin group（1.26±0.31）,deduhonghua-7powde group（1.32±0.37）;DMT1 mRNA:blank group（0.62±0.17）,model group（0.83±0.17）,silybin group（0.43±0.08）,deduhonghua-7powder group（0.43±0.14）;FTMT mRNA:blank group（1.12±0.24）,model group（0.76±0.20）,silybin group（0.79±0.14）,deduhonghua-7 powde group（1.10±0.23）.Compared with blank group,DMT1 mRNA expression in model group was up-regulated,GPX4 and FTMT mRNA expression was down-regulated,with significant differences（P<0.05）.Compared with model group,DMT1mRNA expression was down-regulated in silybin group and deduhonghua-7 powder group;GPX4 and FTMT mRNA expression were up-regulated in deduhonghua-7powder group and silybin group,and there were significant differences（P<0.05）.Conclusion:Mongolian medicine Deduhonghua-7 Powder has protective effect on chronic liver injury induced by CCl₄,and its mechanism is related to reducing iron accumulation in liver tissue and regulating the mRNA expressions of GPx4,DMT1 and FTMT.