Regulation Of Hepcidin On Ferroportin 1, Divalent Metal Transporter 1, Ceruloplasmin And Their MRNA

A large number of researches have demonstrated that some neurodegenerative disease,for example,Alzheimer's disease,Pankinson's disease,was attributed closely to brain iron overload. Significant progress has been made in recent years in iron and iron related protein.However, many questions remain unanswered,for example,the maintenance of brain iron homeostasis and the relationship between iron regulation protein and neurodegenerative disease.Especially we just have a little knowledge about new iron regulation proteins,Hepcidin(Hep).Therefore more researches needed to understand the function of Hep and its interaction with other proteins related to iron.On another hand,more knowledge of Hep will help us to find the way to treat and/or prevent anemia or neurodegenerative disease.purposeTo study expression of Ferroportin 1(FP1),Divalent metal transporter 1(DMT1), Ceruloplasmin(CP)and their mRNA respectively regulated by Hep.MethodThirty-six male Wistar rats were randomly divided into 6 groups:FP1 and FP1 control Group,DMT1 and DMT1 control group,CP and CP control group.FP1,DMT1,and CP group received a Hep microinjection and FP1,DMT1,and CP control group received a saline microinjection.Rats were killed after microinjection 12 hours and the expression of FP1,DMT1,CP in the cortex and hippocampus were showed by immunohistochemistic staining and their mRNA was measured by RT-PCR analysis.Results1 The expression of FP1 and its mRNA in the cortex and hippocampus significantly decreased as compared with control group.The expression of FP1 was significant decreased from 0.2475 in control rats to 0.1711 in the cortex and from 0.2199 to 0.1609 in the hippocampus(P＜0.01).and FP1 mRNA significantly decreased from 1.0789 to 0.9891 in the cortex and from 1.0086 to 0.9131 in the hippocampus(P＜0.01). 2 The expression of DMT1 and its mRNA in the cortex and hippocampus had a significant change as compared with control group.The expression of DMT1 was increased from 0.2053 in control rats to 0.2580 in the cortex and from 0.2264 to 0.2605 in the hippocampus(P＜0.01). And DMT1(+IRE)mRNA significantly increased from 0.5636 to 0.8642 in the cortex and from 0.7539 to 0.8819 in the hippocampus(P＜0.01).And DMT1(-IRE)mRNA significantly decreased from 0.4336 to 0.3861 in the cortex and from 0.6512 to 0.5272 in the hippocampus (P＜0.01).3 The expression of CP and its mRNA in the cortex and hippocampus significantly decreased as compared with control group.The expression of CP was significant decreased from 0.2734 in control rats to 0.2187 in the cortex and from 0.2681 to 0.2099 in the hippocampus(P＜0.01).and CP mRNA significantly decreased from 0.7797 to 0.5213 in the cortex and from 0.7583 to 0.6584 in the hippocampus(P＜0.01).Conclusions1 Hep could inhibit the expression of FP1,CP and their mRNA and change the expression of DMT1 and its mRNA in brain.2 Hep could modulate the expression of the iron-transport proteins:FP1,DMT1,CP and their mRNA,the regulation of brain iron metabolism by Hep might be more similar to other organs.3 Owing to the complexity of brain iron homeostasis,function of Hep in brain should be furtherly researched.