Effects Of Water Extract From Persimmon Parasitism And Oyster And Kudzu Root Solid Drink On Streptozotocin-induced Diabetes In Mice And Its Mechanism

Objective: To investigate the effects and mechanism of Water extract from Persimmon parasitism and Oyster and kudzu root solid drink(OKRD)of oysters on diabetic mice induced by streptozotocin(STZ).Methods: Adaptability to feed this experiment all mice after three days,in addition to the normal control group(NC group),the rest of the intraperitoneal injection of STZ mice(150 mg/kg)diabetes in mice Model was established,after the success of the building is divided into nine groups of mice,each group of 10,NC group respectively,and the Model group(Model),Metformin group(Metformin),WEPP high dose group(WEPP-H group),the WEPP dose group(WEPP-M group),WEPP low dose group(WEPP-L group),OKRD high dose group(OKRD-H group),OKRD medium dose group(OKRD-M group)and OKRD low dose group(OKRD-L group).In addition to the NC group and the model group,other groups were given metformin(320 mg/kg),high,medium and low doses of WEPP(40,20,10g/kg)and high,medium and low doses of OKRD(15,10,5g/kg),respectively,and the changes of mental state and body weight were recorded and observed during the administration.Fasting blood glucose(FBG)was determined on day 0,7,14 and 21.After 21 days of administration,the blood samples were collected and the serum samples were separated to detect fasting insulin(FINS),triglyceride(TG),total cholesterol(TC),AST and ALT,BUN and Scr.The contents of superoxide dismutase(SOD),malondialdehyde(MDA)and glutathione(GSH)in liver tissues were measured.Pancreas,liver and kidney tissues were also taken for HE staining to observe the histopathological changes.Immunohistochemical method was used to observe the protein expressions of Bcl2 and Bax in the kidney of WEPP mice and caspase-3,NF-B,Bcl2 and Bax in the kidney of OKRD mice.Results:(1)Compared with NC group,the serum levels of FBG,FINS,TC,TG,ALT,AST,BUN and S-cr in model group were significantly increased(P <0.05 or P < 0.01);MDA in liver tissue was significantly increased,SOD and GSH were significantly decreased(P < 0.05 or P < 0.01).The results of HE staining showed that the pancreatic islet cells in the model group were seriously damaged,the cells were irregularly arranged,edema,and the size of nuclei was different.The hepatic lobules were blurred or disappeared,and the hepatocytes were swollen,presenting steatosis and vacuolation,accompanied by a small amount of inflammatory cell infiltration.The glomeruli are enlarged,the renal capsule is compressed,inflammatory infiltration is evident,and some epithelial cells are edema.The expression levels of Bax,NF-B,caspase-3 and Bcl-2 in the model group were significantly increased.(2)Comparison with the model group:1)Serum FBG,FINS,TC,TG,ALT,AST,BUN,S-cr in WEPP and OKRD groups were significantly decreased(P < 0.05 or P < 0.01);MDA in liver tissue was significantly decreased,SOD and GSH were significantly increased(P <0.05 or P < 0.01).2)HE staining results showed that WEPP and OKRD could improve the pathological changes of diabetic pancreas in mice in each dose group,especially in high and medium doses.The pathological changes of liver tissues were improved,and the lobular structure,hepatocyte swelling and blank degeneration degree were all reduced compared with the model group.The swelling,vacuolation and necrosis of renal tissue cells were also improved.3)Immunohistochemical staining showed that Bax,NF-B,caspase-3 protein expression levels were significantly decreased in WEPP and OKRD groups,while bcl-2 protein expression levels were significantly increased.Conclusion:WEPP and OKRD can decrease blood glucose and blood lipid in diabetic mice,improve blood lipid metabolism and enhance the body’s antioxidant capacity.