Research On Microfluidic Chip For Myocardial Markers Detection

With the development of society and the improvement of living standards,people have put forward higher requirements for health,as well as the level of health care.The outbreak of COVID-19 pushed In Vitro Diagnosis(IVD)to the top of the wave.Accurate IVD technology is of great significance for clinical or home diagnosis and treatment.As the most important part of IVD,immunodiagnosis has become the mainstream diagnosis method in medical institutions which occupies a dominant market share and shows an explosive growth trend.With the rise of Micro-Electro-Mechanical System(MEMS)and fluid control technology,The emergence of micro fluidic chips has brought subversive breakthroughs to the fields of immune,molecular,biochemical,and clinical diagnosis.The microfluidic chip has excellent detection performance such as high accuracy,good reproducibility,and high sensitivity.It is also easy to realize miniaturization and portability,which provides new opportunities for IVD.However,the current production of microfluidic chips still has limitations such as difficult processing and high cost,which seriously hinder its industrialization and scale up.To solve the above problems,a capillary-based microfluidic chip with segmented microchannels is developed in this paper.It does not need complex processing technology,reduces technical barriers and processing costs,and combines with laserinduced fluorescence(LIF)to achieve low-cost and high-performance rapid quantitative detection.In this paper,the following two aspects are mainly studied:(1)Design and fabrication of microfluidic chip.Based on the design idea,a microfluidic chip with segmented structure is successfully constructed by using capillary as microchannel.The detection device is set up,with the determination of fluorescence signal select.Afterwards,the package shell and pump tube of the chip are also optimized.And the detection results are upgraded by choosing optimal structure and window shape.(2)Optimize the reaction conditions and evaluate the chip analytical performance.The surface processing conditions of the inner wall of the microchannel and immune conditions are optimized by using Myoglobin(myo)as the analyte model.Finally,the analysis and detection performance results showed that the chip has great specificity,low detection limit,excellent reproducibility and long-term stability.Meanwhile,the detection result as well as the comprehensive performance of microfluidic chip are compared with the commercial general method,which shows that the chip has a bright market prospect.