Studies On Chip-Based Capillary Electrophoretic Enzyme Immunoassay With Electrochemical Detection For Tumor Markers

Microchip-based capillary electrophoresis technologies, which grew in the early 90,s of the 20th century, are being become the research focuses in the modern analytical sciences. This thesis establishes a chip-based capillary electrophoretic electrochemical enzyme-linked immunoassay analysis platform to detect tumor markers. This thesis is divided into seven chapters.The first chapter is an introduction, it simply introduces the Chip-CE, summarizes the detected methods used in Chip-CE. Sensitive and small detector is important for Chip-CE, electrochemical detection is an especially sensitive method, we also discuss the character of enzyme-linked immunoassay, discuss the Chip-CE/electrochemical detection/enzyme-linked immunoassay detect pattern.The second chapter introduces PDMS/glass compound chip, this chip is composed of PDMS chip and glass substratum. This chapter simply introduces the design and the process of PDMS chip and glass substratum, and validates the property of electrode on the glass substratum.The third chapter establishes the technique platform for microchip-based capillary electrophoretic electrochemical enzyme immunoassay, the orthoaminophenol(OAP) -H2O2-horseradis hperoxidase(HRP)enzyme-linked immunoassay system was used, under the optimized conditions, HRP can be measured with linear range of 4.5×10-9 - 7.8×10-13 mol/L, and a detection limit of 1.6×10-13 mol/L (S/N = 3), which establishes the groundwork for followed study.The fourth chapter establishes the detected method for alpha-fetoprotein (AFP) in human serum sample, its high sensitivity is higher than ELAISA method. The linear range is 0.5 - 80.0 ng/mL, detection limits (S/N = 3) was 0.125 ng/mL. Under the optimum conditions, the human serum samples were detected. The results showed good corresponding relationship with those in ELAISA method. The detected speed is rapid, and the sample is saved.The fifth chapter establishes the detected method for carcinoembryonic antigen (CEA) in human serum sample, its sensitivity is higher than ELAISA method. The linear range is 0.5 - 66.0 ng/mL, detection limits (S/N = 3) was 0.25 ng/mL. Under the optimum conditions, the human serum samples were detected. The results showed good corresponding relationship with those in ELAISA method. The detected speed is rapid, and the sample is saved.The sixth chapter establishes the detected method for prostatespecific antigen (PSA) in human serum sample, its high sensitivity is higher than ELAISA method. The linear range is 1.0 - 70.0 ng/mL, detection limits (S/N = 3) was 0.20 ng/mL. Under the optimum conditions, the human serum samples were detected. The results showed good corresponding relationship with those in ELAISA method. The detected speed is rapid, and the sample is saved.The seventh chapter establishes the detected method for AFP and CEA in human serum samples with rapid detected speed and good separate.