Study On Anti-Tumor Material Basis Of Xiaoai Jiedu Decoction Ⅰ

Xiaoai Jiedu Decoction I was established by Professor Zhou Zhongying,a master of Chinese medicine,based on the theory of cancer and the clinical practice.Among them,Xiaoai Jiedu I Decoction(Oldenlandia diffusa,Scutellariae barbatae herba,Cremastrae Pseudobulbus Pleiones Psedobulbus,Pseudostellariae Radix,Curcumae Rhizoma,Ophiopogonis Radix)is used to treat colorectal cancer in the digestive system.In this paper,the chemical constituents,pharmacological effects,and tissue distribution of Xiaoai Jiedu I Decoction were studied.In order to study the material basis of the compound,the chemical composition of the single-flavored drug was qualitatively analyzed by HPLC-ESI-Q-TOF-MS technique.LC-MS/MS and reverse silica gel columns were used,eluted with acetonitrile-0.1%aqueous formic acid as mobile phase,mass spectrometry using ESI ion source,and data collected in positive and negative ion mode.A total of 48 compounds were identified by ion mass spectrometry information and elemental composition in comparison with related literature.The chemical constituents in the single-flavored aqueous extract were analyzed,and the results of the analysis of the components of the compound aqueous extract were compared.Changes in chemical composition before and after compound compatibility were found.Column chromatography coupled with modern spectral analysis techniques was used to separate thirteen monomers from the ethanol extraction site of Scutellaria barbata.In the study of the tissue distribution of the compound,the liquid-mass spectrometry method was used to study the distribution of rutin,wild baicalin and scutellaria B in the tissues of mice.The results showed that rutin and hemiphyllin B was widely distributed in mice,and the content of wild baicalin was relatively low in tissues;rutin was mainly distributed in the liver,followed by kidney and spleen,and the lung was the least distributed;wild baicalin was only detected in the lung;The alkaloid B was mainly distributed in the liver and kidney,followed by the heart and lungs,and the spleen was the least.4’-hydroxywogonin was separated by column chromatography coupled,as a test drug to interfere with the growth of colon cancer cells by IL-6 inflammatory cytokines.Proved by western blot assay and cell migration assay,the results indicated that 4’-hydroxyhanzein inhibited tumor angiogenesis by inhibiting the expression of VEGF in colon cancer cell HCT116 by blocking the PI3K/AKT signaling pathway,thereby inhibiting tumor growth.