Study On The Relationship Between Vaginal Microecology And Thl/Th2 Subsets Of Cervical Canal And High-risk HPV Infection

Objective To explore the relationship between vaginal microecology and Th1/Th2 subsets of cervical canal and high-risk HPV infection,to provide a theoretical basis for correcting the adverse vaginal environment of high-risk HPV positive patients in genital tract and improving the negative conversion rate of high-risk HPV.Methods From March 2018 to March 2019,60 high-risk HPV positive patients aged 18-35 years were selected as the observation group,sixty healthy women of the same age with a history of sexual life were taken as the control group.The vaginal microecology of two groups of female vaginal secretions was analyzed.The secretion of cervical canal was extracted and cytokines were detected by human Th1/Th2 subgroup detection kit(flow cytometry).IL-2 in Th1 family and IL-10 in Th2 family were selected as observation indexes(IL-2/IL-10).The correlation between vaginal microecology and Th1/Th2 subsets of cervical canal and high-risk HPV infection was evaluated.Results 1.There was no difference in the general situation between the two groups(P>0.05),which was comparable.2.The bacterial vaginosis,abnormal cleanliness of leucorrhea,alkaline vaginal PH value,positive leucocyte esterase and hydrogen peroxide were compared between the two groups(P<0.05).3.The abnormal rate of TCT was different between the two groups(P<0.05).4.IL-2 value:The observation group(HPV positive)was 21.693±4.985 pg/ml and the control group(HPV negative)was32.868±5.328 pg/ml.There was significant difference between the two groups(P<0.01).5.IL-10 value:The observation group(HPV positive)was 30.448±5.496 pg/ml and the control group(HPV negative)was 24.729±4.251 pg/ml.There was significant difference between the two groups(P<0.01).6.IL-2/IL-10 value:The observation group(HPV positive)was 0.737±0.231 pg/ml and the control group(HPV negative)was 1.366±0.310 pg/ml.There was significant difference between the two groups(P<0.01).7.In the observation group,with the aggravation of cervical lesions,IL-2 showed a downward trend,IL-10 showed a gradual upward trend and IL-2/IL-10 showed a gradual downward trend.Conclusion 1.High-risk HPV infection is associated with vaginal microecological abnormalities such as bacterial vaginosis,abnormal leucorrhea cleanliness,vaginal pH alkalinity,leukocyte esterase and hydrogen peroxide positive.2..High-risk HPV infection is associated with abnormal TCT.3.HPV infection and cervical lesions were negatively correlated with IL-2,positively correlated with IL-10 and negatively correlated with IL-2/IL-10.