Effects Of Leptin On Frozen Preservation Of Dairy Goat Semen

Saanen dairy goat has the advantages of strong adaptability and high milk yield,which can improve the economic benefits of farmers.Large-scale and intensive breeding is the mainstream mode of dairy goat breeding in the future.Referring to the development of dairy cattle breeding industry,frozen semen artificial insemination will also become an important technical support for the rapid propagation of dairy goat breeds in the future.In the process of artificial insemination of frozen semen,the quality of sperm directly affects the efficiency of conception.Studying how to improve the quality of frozen semen after thawing plays an important role in promoting the overall breeding efficiency of large-scale dairy goat farms and accelerating the process of group improvement.Leptin(LEP)is a polypeptide hormone that can improve the quality of frozen semen by reducing the production of reactive oxygen species(ROS)during cryopreservation.In this study,different concentrations of LEP(0ng/m L,5ng/m L,10ng/m L,15ng/m L,20ng/m L)were added to the cryoprotectant,and the best concentration of LEP was selected by detecting the sperm quality of dairy goats after freezing and thawing.The antioxidant capacity and quality of frozen semen were detected after freezing-thawing,and the effect of LEP on the cryopreservation of semen of Saanen dairy goats was analyzed.The main results of this study were as follows :1.Adding different concentrations of LEP to the cryoprotectant of Saanen dairy goat semen can significantly increase the T-AOC content in frozen thawed semen,reduce ROS levels,MDA content,and DNA fragmentation rate(P<0.05).2.Addition of different concentrations of LEP in semen cryoprotectant can significantly improve the sperm motility,the rate of plasma membrane integrity,the rate of acrosome integrity and the mitochondrial membrane potential difference after freezing thawing(P<0.05).3.In the Saanen dairy goat semen cryoprotectant,LEP can significantly improve the blastocyst rate of in vitro fertilization(P<0.05).Conclusion: Adding 10ng/mL and 15ng/mL LEP to frozen semen can significantly reduce the oxidative stress damage caused by semen freezing-thawing process,and improve the motility,plasma membrane integrity,acrosome integrity and mitochondrial membrane potential of frozen semen after thawing.Adding LEP frozen sperm for in vitro fertilization can significantly improve blastocysts.