Effects Of HSPA8 And Sp Addition Before Freezing And After Thawing On Sperm Quality

Since boar sperm is not as freezing as other animals,it has not been cryopreserved in the commercial field.This study aims to evaluate the effects of adding HSPA8 before freezing and adding seminal plasma after thawing on the quality of boar semen.In this experiment,the pseudovaginal method was used to collect Landrace pig semen,and the boar semen cryoprotectant with a concentration of 0.5 μg/ml HSPA8 was added for thin tube packaging.The boar semen is frozen and stored in liquid nitrogen for 3 weeks and then thawed.After thawing,adding different concentrations of seminal plasma(10%,30%,50%)will affect the seminal plasma membrane integrity,acrosome integrity,apoptosis,mitochondrial membrane potential and protamine of Landrace boar sperm after freezing and thawing.influences.Lack of technical aspects,such as in vitro energy capture levels and other technical evaluations.Objective To explore the effects of adding HSPA8 before freezing and adding different concentrations of seminal plasma after thawing on frozen-thaw boar sperm,and to provide certain technical support for the cryopreservation and commercial production of boar sperm.The test results are as follows:1.Compared with 3% glycerol in the control group,when 0.5μg/ml HSPA8 is added and seminal plasma is not added to the thawing dilution,sperm motility parameters,plasma membrane integrity,acrosome integrity,mitochondrial membrane potential and apoptosis are other Parameters were significantly increased(P <0.05),sperm WOB and Lin were not significantly different(P> 0.05);sperm and protamine deletion rate was significantly reduced(P <0.05);sperm tyrosine phosphorylation level was significantly increased(P <0.05)).2.Compared with the group without seminal plasma,when the seminal plasma concentration was 30%,sperm motility,plasma membrane integrity,acrosome integrity,and mitochondrial membrane potential began to increase significantly(P<0.05).When the seminal plasma concentration was 50%,sperm motility and LIN also began to increase significantly(P<0.05).When the seminal plasma concentration is 50%,the improvement effect is the best,which is significantly higher than that of the control group(P<0.05).3.Compared with the group,there is no thawing and seminal plasma dilution.This is to find that when the concentration of seminal plasma is 30%,the level of sperm protamine begins to decrease significantly(P<0.05),and it is the concentration of the most seminal plasma when the effect is improved.The best group is 50%,and significantly higher than the control group(P<0.05).4.Compared with the 3% glycerol control group,the treatment group with 0.5μg/ml HSPA8 added to the thawing dilution without the addition of seminal plasma and the treatment group with different concentrations of seminal plasma added to the thawing dilution with sperm protein tyrosine phosphate The chemical level was significantly improved(P<0.05).There was no significant difference between adding 0.5μg/ml HSPA8 to thawing,without adding seminal plasma dilution and adding 10% seminal plasma thawing dilution(P> 0.05)for the treatment group,adding 50%sperm protein tyrosine phosphate to the treatment and seminal plasma The chemical level group was significantly higher than the other test groups(P <0.05).The above results show that adding HSPA8 to the cryoprotectant and adding seminal plasma to the thawing diluent can significantly improve sperm motility,plasma membrane integrity,acrosome integrity,mitochondrial membrane potential and cell apoptosis when porcine sperm is cryopreserved.The death level and protein tyrosine phosphorylation level,especially when the amount of seminal plasma added reaches 50%,has a more significant impact.This study has improved the cryoprotectant of boar semen,which can provide certain commercial value for the freezing of boar semen and the preservation of improved varieties.