Development Of T1AS.1M~g Translocation Lines Carrying 1M~g Glutenin Gene

Aegilops geniculata Roth（Aegilops geniculata Roth,2n=4x=28,U^gU^gM^gM^g）represents a source of potentially useful genetic variation of relevance to bread wheat improvement,its high molecular weight glutenin subunits on 1M^g chromosome are valuable in wheat breeding.In this study,screening population and markers were developed to obtain to wheat translocation line carrying 1M^g glutenin gene by centric breakage and fusion.The main results are as follows:1.The 1M^g and 1A centric breakage and fusion translocation lines screening population[CSN1AT1B/（add L-1/CSph1b）F₂]was constructed by hybridization and molecular marker selection.2.Referring to the homologous gene sequence of PLUG marker,6 KASP markers on short arm of 1M^g（0.44M on 1A）and 2 KASP markers on long arm of 1M^g（381.4M on 1A）were developed.1 KASP markers of 1Mx was also developed.3.Molecular identification showed that four plants（B1,B9,A1 and A22）carried only 1M^gL and 1AS chromosomes,and their seeds of transmissibility of 1My HMW-GS were 40%-48%respectively,1AS.1M^gL translocation may have occurred in these plants.A1 has homozygous ph1b and can be used to screen small fragment translocation of 1M^gL in next generation.In addition,there are another 2 plants（A15and A18）have homozygous ph1b,their seeds may have small fragment translocation of 1Mg L.4.T1AS.1M^gL translocation lines A1-65 was identified by FISH karyotype.