Effects Of Pineapple Enzyme On Growth Performance,Immune Function And Intestinal Morphology Of Broilers Under Heat Stress

To investigate the effects of pineapple enzyme on growth performance,immune function and intestinal morphology of white-feathered broilers under heat stress,105one-day-old healthy white-feathered broilers with consistent body condition were randomly divided into 3 treatments with 5 replicates per treatment and 7 broilers per replicate.The control group was fed basal diet and normal drinking water at(35±1)℃.On the basis of control group,test group I was supplemented with 0.1% pineapple enzyme in drinking water.On the basis of the control group,test group II was supplemented with 0.2% pineapple enzyme in drinking water.The experiment lasted for 42 d.The results showed as follows:under heat stress,compared with the control group,the average daily feed intake(ADFI),average daily gain(ADG)and feed/gain(F/G)of white feathered broilers were not significantly affected by test group(Ⅰ P>0.05);There were no significant differences in the apparent digestibility of crude protein(CP),Ash(Ash)and crude fiber(CF)of white broilers(P>0.05).The contents of lactate dehydrogenase(LDH),blood glucose(GLU),alkaline phosphatase(ALP),glutamico-oxalacetic transaminase(AST),serum total bilirubin(T-Bil),total protein(TP),total cholesterol(TC),triglyceride(TG),UREA(UREA),albumin(ALB),alanine aminotransferase(ALT)and so on in serum of white broilers were studied There were no significant effects on the indexes(P>0.05).However,group significantly increased the Ⅰcontents of immunoglobulin A(Ig A),immunoglobulin G(Ig G)and immunoglobulin M(Ig M)in serum of white feathered broilers(P<0.01),and improved the apparent digestibility of crude fat(EE)of white feathered broilers(P<0.05).During 1-28 days,compared with the control group,the contents of total antioxidant capacity(a-TOC)and catalase(CAT)in serum of white feather broilers in test group were Ⅱsignificantly increased(P<0.05),and the content of malondialdehyde(MDA)in serum was significantly decreased(P<0.05).From 28 to 42 days,compared with the control group,the serum T-AOC,superoxide dismutase(SOD)and CAT in test Ⅱgroup were significantly increased(P<0.05),and the serum MDA content was extremely significantly decreased(P<0.01),but there was no significant difference between test group and other groups.During the whole experiment period(1-42d),compared with other groups,the contents of T-AOC,SOD and CAT in jejunum and ileum of white feather broilers in test Ⅱgroup were significantly increased(P<0.01).Compared with the control group,the contents of glutathione peroxidase(GSH-Px)in jejunum and ileum of white feather broilers in test Aln and Aln groups were significantly increased(P<0.01).The content of MDA in jejunum and ileum was significantly decreased(P<0.01),and the effect of Ⅱgroup was significantly better than that of other groups.The results showed as follows: test Ⅱgroup significantly increased the activities of amylase and lipase in jejunum of white feathered broilers(P<0.01),and significantly increased the activity of sucrase in jejunum of white feathered broilers(P<0.05).Compared with the control group,the activities of amylase,lipase,trypsin and sucrase in ileum of white feathered broilers were not significantly affected by experiment and supplemented group Ⅰ(P>0.05),but the activities of lactase and maltase in ileum of white feathered broilers were significantly increased(P<0.05).Compared with the control group,pineapple enzyme could reduce the rectal temperature of white broilers,and the effect of group was significantly Ⅱbetter than that of the test group(P<0.01).The results showed that compared with the control group,the crypt depth(CD)of jejunum and ileum of broilers was not significantly affected by trial and PA group(Ⅰ P>0.05).The villus height(VH)and VH/CD of jejunum and ileum of white broilers in group were significantly increased(Ⅱ P<0.05).according to the above,the heat stress condition,the oxidation resistance of white chicken pineapple enzyme,the immune function and improve animal gut health effect is remarkable,to add 0.2% water level of the pineapple enzyme effect is best.