Effects Of Potato Starch Processing Juice Water On Soil Fertility And Yield And Quality Of Alfalfa

In order to explore the effect of potato starch processing juice on soil fertility and yield and quality of alfalfa,and provide basic data for its waste resource utilization.In this study,a single-factor experiment in the field was used,and alfalfa was used as the test crop.Five irrigation gradients(300,225,150,75,0 m3/hm2)were set up for the processing of potato starch,and the equal irrigation method was adopted.The total amount of irrigation water for the treatment was set to 300 m3/hm2,and the irrigation amount less than 300 m3/hm2 was supplemented with clear water to explore its effects on soil environment alfalfa growth,yield and quality,alfalfa leaf metabolism and protein,according to the measurement indicators to determine the optimal irrigation amount,in order to provide a theoretical basis for the utilization of potato starch processing juice to field irrigation waste resources.The main findings are as follows:(1)Irrigation of potato starch processing juice can generally improve soil fertility and bacterial community structure and function as well.By analyzing the impact of different irrigation amount of potato starch processing juice on alfalfa soil bacterial community structure,it is to provide a theoretical basis for its resource utilization.Five irrigation gradients of potato starch processing juice(300,225,150,75,0 m3/hm2)were set up respectively,and the equal irrigation method was adopted,that is,the total amount of irrigation water for each treatment was set to 300 m3/hm2,when less than 300 m3/hm2,it was supplemented with clean water.The results showed that compared with non-irrigated juice treatment,potato starch processing juice irrigation could significantly increase soil conductivity,organic matter,alkali-hydrolyzed nitrogen,available phosphorus and available potassium content(P<0.05),and reduce soil pH value;different treatment of irrigation amounts of potato starch processing juice irrigation compared with MI5,soil bacterial community species richness and diversity indices of Chaol,ACE,Shannon and Simpson decreased significantly(P<0.05),with the increasing of potato starch processing juice irrigation amount it increased first and then decreased slightly;the bacterial community species distribution indicating that the relative abundance of the dominant phyla of Proteobacteria and Actinobacteria were gradually decreased,the relative abundance of dominant genera of Sphingomonas decreased,while RB41 and MND1 increased;for all the samples of treatment and MI5,the bacterial community structure in between them was significantly different(P<0.05),the significant phyla were Bacteroidetes and Acidobacteriaceae,the significant different genera were Halophila,Bacteroides,Shigella,and Galella;the important environmental factor driving community structure changes was soil conductivity;soil community species function annotation reveled that soil bacterial communities had 6 types of primary metabolic pathways and 44 types of secondary metabolic pathways.(2)Potato starch processed juice could promote soil nutrient accumulation and alfalfa growth in a concentration-dependent manner.Compared to the values of control plots,under a juice irrigation amount of 300 m3/hm2,fresh alfalfa yield,hay yield,chlorophyll content,and crude protein content increased by 160.29%,165.31%,29.61%,and 6.97%,respectively.Moreover,at an irrigation amount of 225 m3/hm2,the height of alfalfa plants increased by 38.06%and the relative feeding value by 36.54%.Finally,at an irrigation amount of 75 m3/hm2,the crude ash content was reduced by 3.25%and the crude fat content was increased by 0.68%.By using principal component analysis and integrating data on soil nutrients and plant properties,an irrigation amount of 300 m3/hm2 was deemed to be optimal.(3)Potato starch processing juice irrigation has a significant effect on metabolites of alfalfa leaves.There were 5118 metabolites that were significantly different between the irrigated potato starch processing juice and the non-irrigated group,of which 2203 were up-regulated and 2915 were down-regulated.Differential metabolites were annotated in the KEGG database to obtain 31 differential metabolic pathways,included phenylalanine metabolism,flavonoid and flavonol biosynthesis,alanine,aspartate and glutamate metabolism,glycolysis or gluconeogenesis Biogenesis and pyruvate metabolism were significantly enriched metabolic pathways(P<0.05).L-Phenylalanine,4-Hydroxycinnamic acid,Kaempferol,Luteolin,L-Glutamic acid,L-Asparagine and S-Acetyl dihydrolipoamide were used in alfalfa irrigation potato starch processing juice the main product.(4)Potato starch processing juice irrigation has a significant effect on protein production of alfalfa leaves.A total of 4818 proteins were detected,of which 4768 were quantifiable.Among them,70 were up-regulated and 91 were down-regulated.Through GO analysis,it was found that after irrigating potato starch to process juice,the differential proteins were mainly in cellular processes,metabolic processes,cells,intracellular,catalytic activity and binding.The differential proteins were mainly located in the chloroplast and cytoplasm;the differential proteins were mainly enriched in the Sugar and glucuronide interactions,linoleic acid metabolism,alpha linoleic acid metabolism,amino sugar and nucleotide sugar metabolism,fatty acid degradation,phenylpropane biosynthesis,and flavonoid biosynthesis pathways.