Debao And Landrace Pigs Intermuscular Adipose Tissue MRNA Transcriptome Sequencing And Analysis

Debao pig is known as one of the seven excellent breeds in Guangxi.Compared with the imported foreign pig breeds,its meat quality is more satisfying to consumers’ taste.Many studies have shown that the expression of differential genes is closely related to the differences between pig breeds.Therefore,using the transcriptome levels to explore the expression of differential genes can provide a better interpretation of the reasons for the differences in pig germplasm.In this study,excellent local pig breeds Debao pig(DB)in Guangxi and foreign introduced pig breed Landrace(CB)were selected as experimental animals.Using high-throughput transcriptome sequence(RNA-seq)technology,transcriptome sequence and biological information analysis of intermuscular fat in Debao and Landrace pigs,functional annotation and pathway enrichment analysis of the differential genes were analyzed.This study analyzed the differences in the variable shear of adipose tissue between two different pig breeds,protein interaction networks,and m RNA-mi RNA targeted interaction networks.Besides,this study randomly selected 5 genes for real-time fluorescence quantitative PCR verification,the verification results are basically consistent with the sequence results.The main results obtained in this study are as follows:1)In the high-throughput sequencing of intermuscular adipose tissue of Debao and Landrace,the number of clean reads obtained by Debao intermuscular fat tissue was 5,956,000,and the number of clean reads of Landrace was 5,554,000;and clean after filtering.The quality value of reading Q20 value is more than 97%,Q30 value is more than 85%;more than 90% of sequencing reads can be compared to the pig genome.2)The setting range log2FC>=2,Qvalue<=0.001 is differentially expressed genes,a total of 956 different genes were screened,including 606up-regulated genes and 350 down-regulated genes.A total of 9,135 transcripts were screened,with 4,670 up-regulated and 4,465 down-regulated.3)In the gene expression level,the number of genes unique to the sample of Landrace is 483,the number of genes unique to the Debao pig is 647,and the number of genes intersected by the two breed samples is 16,535.4)The set screening range is q<0.05,and 229 differential genes were screened for 956 different genes for fat deposition,fat metabolism,and fat development.Enrich analysis of signal pathways and gene ontology(GO)of 229 differential genes.The differentially expressed genes were mainly enriched in metabolic pathways,arachidonic acid metabolism,and Wnt signaling pathways.The main biological processes involved were DNA template,positive regulation of RNA polymerase II transcription,positive regulation of transcription,adipocyte differentiation process.5)A total of 5 variable shear types were detected in this study,of which the skipped exon(SE)variable shear type accounts for the largest proportion,accounting for 41%.6)In the protein interaction network analysis,we screened for six candidate key genes that potentially regulate intermuscular fat deposition.In the constructed m RNA-mi RNA targeting interaction network,there are 158 mi RNAs that are targeted to m RNA,and all of them are newly predicted mi RNAs.In summary,this study screened the differentially expressed genes of two different pig breeds through RNA-seq technology,and conducted a deeper analysis of the differentially expressed genes to discover the key signal pathways of the two breeds of pigs in terms of fat and meat quality.As well as the key genes annotated in the signaling pathway,it provides a reference to the study of the intramuscular fat deposition mechanism of the local Debao pig in Guangxi,and provides a theoretical basis for the development of the Debao pig.