Screenning Of Resource Resistant To Sclerotinia Sclerotroium In Oilseed Rape

Sclerotinia sclerotiorum caused by fungal pathogen Sclerotinia sclerotiorum(Lid)de Bery is a soil-borne fungal disease that affects crop growth.Sclerotinia disease is the most serious disease among the three major diseases of rapeseed,which has long restricted the growth of rapeseed and the development of the industry.It is particularly serious in the main producing areas of winter rape in northern China.At present,no high-resistance or immunized varieties have been found in disease-resistant rapeseed breeding and its related species.At the same time,the lack of accurate and reliable identification methods has led to slow progress in the breeding of antibacterial nuclear diseases in rapeseed,and it has found an accurate identification method to reveal rapeseeds.The antibacterial nuclear disease mechanism of the varieties is of great significance in the breeding of high-quality,disease-resistant rapeseed varieties.In this study,240 accessions of winter rapeseed germplasm provided by the research group were used to screen for antibacterial nuclear diseases.In the first flowering period and the final flowering period of rapeseed in 2017,field stalk inoculation method(mycelium leaf inoculation method)was used for resistance identification.Some of the resistant germplasms were screened in 2017 for further disease resistance identification.In the early flowering period of rapeseed in 2017,field tests were conducted using the field stalk toothpick puncture method to verify the results of the 2017 screening.The main results are as follows:1.In 2017,the rapeseed stalk inoculation method(mycelium leaf and leaf inoculation method)was used for the initial flowering and final flowering of rapeseed in 2017.The sclerotiorum resistance was identified in 240 materials collected and selected by the research group,and the material was finally classified as high.In the five groups of resistance,medium resistance,low resistance,low sensation,and high sensation,2 high resistance materials,2 medium resistance materials,13 low resistance materials,25 low performance and 198 high sensitivity screening were selected.It accounts for 1.00%,1.00%,5.00%,10.00%,and 83.00%of the tested materials,respectively,indicating that most of the materials are susceptible materials.The two materials obtained in this study have theoretical and practical significance for the study of the mechanism of antibacterial nuclear diseases in rape and the creation of rapeseed germplasm and breeding of resistant varieties.2.Comparison of resistance identification methods.Three different methods for the identification of resistance were used in this study.They were inoculation of leaf mycelia at the seedling stage,identification of in vitro leaf at the seedling stage of mycelial agar,and indentation identification of the field stem stalk toothpick at the early flowering stage.The comparative analysis of these resistance identification methods found that these resistance identification methods can significantly distinguish the material’s anti-inductive susceptibility.There are extremely significant correlations between the two identification methods at the seedling stage,potted leaf mycelium leaf resistance identification method and seedling stage bacteria.The silk agar block in vitro leaf inoculation identification method was very significantly related to the initial flowering period of the stalk toothpick inoculation identification method.In summary,it can be concluded that the results of inoculation of the three different identification methods are highly positively correlated.In contrast,the correlation coefficient(0.9198)between the inoculation method of leaf seedlings at the seedling stage and that of the field toothpick stalk stalk inoculation method was higher than that of the inoculation method of the in vitro leaf mycelia inoculation method and the field toothpick stalk puncture inoculation method.The correlation coefficient(r=0.871),based on the puncture inoculation of toothpicks in the field,is more in line with the actual incidence.Therefore,it is believed that the identification of Sclerotinia by the inoculation of leaves in the seedling stage of indoor pots is more reliable,and the operation is relatively simple and takes up space.Small,easy to control the disease environment;However,the in vitro leaf mycorrhizal inoculation method can be combined with field planting,take leaves for resistance identification,eliminating the process of pot seedlings,the method is more simple,the results are more reliable,can be used The initial screening of a large number of materials.