| Objective:To study the demographic data,disease-related factors,TCM Syndrome Distribution and TCM syndrome characteristics of chronic atrophic gastritis(CAG)with low-grade intratumoral transformation;Objective to study the pathological situation of chronic atrophic gastritis with low-grade intratumoral transformation and the distribution of atrophy and intestinal metaplasia in corresponding parts;At the same time,the relevant cell experimental research,so that the research results can better provide a useful scheme for clinical treatment.In addition,this project studies the anti-inflammatory effect of Shenbai granule at the cellular level,which provides a research basis for its clinical application and a reference for the later clinical research.Reserch methods:The clinical information of 150 cases of chronic atrophic gastritis with low-grade neoplasia were collected and analyzed.The basic information,personal medical history and family history,pathological examination results of each patient were collected in the form of questionnaire to determine the TCM syndrome classification.CCK8 method was used to monitor the cytotoxicity of Shenbai granules.The effect of Shenbai Granule on inflammatory factors was determined by PCR.Spss.22 software was used for descriptive statistics,chi square test was used for count data,t test or rank sum test was used for measurement data.P<0.05 means there is statistical difference.Results:A total of 150 patients were collected in this study.After investigating their general data,it was found that male patients accounted for 50.7%and female patients 49.3%.The oldest patient was 78 years old,the youngest patient was 34 years old,and the average age was 56.84±8.94 years old.Chi square test was used to compare the proportion distribution of men and women in different age groups.The results showed that there was no significant difference(P>0.05).Among them,patients engaged in mental work accounted for 66.7%,and patients engaged in physical work accounted for 33.3%.The mean BMI was 22.68 ± 2.53.Among them,the proportion of patients with normal BMI is the highest,followed by overweight,and the proportion of thin and obese patients is not high.Among them,patients from the North accounted for 98.7%and patients from the South accounted for 2.3%.The proportion of patients with warm living environment is the highest,followed by dry.In addition,the proportion of patients with humid,cold and hot living environment is relatively low.Among them,53.3%of the patients had the first attack;In addition,the incidence of recurrence accounted for 46.7%.The proportion of patients whose first onset season was autumn was the highest;Followed by winter;The proportion of patients whose first onset season was summer was the lowest.The average number of recurrence was 2.81 ± 1.44.The highest proportion of recurrence was 2;The second is 5 times;The lowest proportion of recurrence was 4.Among them,the proportion of patients with cold is the highest;The second is the autumn and winter season;The proportion of patients with fever is the lowest.Among the patients with recurrence,the proportion of treatment time more than 3 months was the highest;The proportion of patients with treatment time less than 1 month and 1-3 months is relatively low,accounting for 16.0%and 19.3%respectively.The proportion of patients who choose integrated traditional Chinese and Western medicine is the highest;The second is traditional Chinese medicine;The proportion of patients who choose Western medicine as the treatment method is the lowest.The proportion of patients with postprandial pain was the highest;Secondly,no pain;The proportion of patients with pre meal pain and continuous pain is relatively low.The proportion of patients with flatulence pain was the highest;The second is dull pain;The proportion of patients with stinging stomach pain was the lowest.The proportion of patients with gastric distention before meal was the highest;The second is continuous inflation;The proportion of patients with and without gastric distention after meal is relatively low.The patients with acid reflux noise accounted for 66.7%;In addition,33.3%of the patients did not have acid reflux noise.The patients without nausea accounted for 58.7%;In addition,patients with nausea accounted for 41.3%.Belching is sometimes the highest in some patients;The second is no;Belching is frequent,and the proportion of patients is the lowest.The proportion of patients with hypochondriac pain was the highest;The second is lasting for 1-3 hours;The proportion of patients with costal pain lasting more than 3 hours was the lowest.62.0%of the patients had pain changing with emotion;In addition,patients whose pain did not change with emotion accounted for 38.0%.The proportion of patients with slight abdominal distension after eating was the highest;Followed by 1-3 hours;The proportion of patients without postprandial abdominal distension was the lowest.The proportion of patients without fear of cold and heat was the highest;Secondly,fear of cold;The proportion of patients who are afraid of heat is the lowest.The proportion of patients whose physical state is sometimes weak is the highest;Secondly,energetic;The proportion of patients with frequent fatigue was the lowest.The proportion of patients with general appetite was the highest;The second is lack of appetite;The proportion of patients with very good appetite was the lowest.The proportion of patients with normal oral taste and bitter mouth was the highest;Secondly,it has no taste;The proportion of patients whose oral taste was sticky was the lowest.The patients with regular stool accounted for 91.3%;In addition,patients with irregular stool accounted for 8.7%.The proportion of patients with normal stool texture was the highest;Secondly,it is relatively dry;The proportion of patients whose stool texture was not formed was the lowest.The patients with normal stool taste accounted for 84.0%;In addition,the proportion of patients with strong stool taste accounted for 16.0%.The proportion of patients with light yellow urine was the highest;Secondly,the color is very yellow;The proportion of patients with clear and unclean urination is relatively low.The proportion of patients who were prone to anger was the highest;Secondly,emotional peace;The proportion of patients with optimistic emotional situation was the lowest.The proportion of patients with general sleep was the highest;The second is relatively poor;The proportion of patients with very poor sleep was the lowest.The proportion of patients with abdominal distension was the highest;Followed by abdominal pain;The proportion of patients with low back pain was the lowest.The proportion of patients with fat tongue shape and texture was the highest;The second is thin;The proportion of patients with large tongue shape and texture was the lowest.The proportion of patients with thin and greasy tongue coating was the highest;The second is thick and greasy;The proportion of patients with moist tongue coating was the lowest.The proportion of patients with yellow tongue coating was the highest;The second is white;The proportion of patients with yellowish tongue coating was the lowest.According to the analysis of other Western medical examination parameters,patients with anemia accounted for 4%;The patients with family history of gastropathy accounted for 19.3%;Patients with other intestinal diseases accounted for 6.7%;Patients with other diseases other than digestive tract accounted for 21.3%;Helicobacter pylori infection accounted for 4.0%.The order of TCM syndromes from high to low is weakness of spleen and stomach>blood stasis of gastric collaterals>stagnation of liver and stomach qi>damp heat of spleen and stomach>stagnation of liver and stomach heat>deficiency of stomach yin.The order of single TCM syndrome from high to low is weakness of spleen and stomach>blood stasis of gastric collaterals>stagnation of liver and stomach qi>stagnation of liver and stomach heat>damp heat of spleen and stomach>deficiency of stomach yin.The order of the two TCM syndromes from high to low is spleen and stomach weakness+spleen and stomach damp heat>deficiency of stomach yin+blood stasis in gastric collaterals>Blood Stasis in gastric collaterals+liver and stomach qi stagnation>Blood Stasis in gastric collaterals+liver and stomach qi stagnation>spleen and stomach weakness+liver and stomach qi stagnation.The order of Three TCM syndromes from high to low is spleen and stomach weakness+liver and stomach qi stagnation+liver and stomach stagnation heat>spleen and stomach weakness+stomach collateral blood stasis+spleen and stomach damp heat>spleen and stomach weakness+stomach collateral blood stasis+liver and stomach qi stagnation>spleen and stomach weakness+stomach yin deficiency+stomach collateral blood stasis.The order of the site of internal neoplasia from high to low is gastric antrum>gastric horn>gastric body>junction of gastric body and antrum>anterior pyloric area.The distribution of TCM syndromes in different parts of neoplasia is similar to that in the whole.In the cytological experimental study of Shenbai granules,gastric mucosal cells were treated with different doses of Shenbai granules for 24 hours and 24 hours.The experimental results showed that 50 μ Shenbai granules with the concentration below g/ml can maintain the cell survival rate of more than 90.0%.The effect of Shenbai Granule on the content of iNOS and COX-2 was monitored by PCR.The experimental results showed that the expression of iNOS and COX-2 in cells treated with LPS increased significantly.The expression of iNOS and COX-2 in cells treated with Shenbai granule decreased significantly in a dose-dependent manner.Conclusion:In this study,a total of 150 patients were collected for investigation,and the ratio of male to female was 1:1.The oldest patient was 78 years old,the youngest was 34 years old,the average age was 56.84±8.94 years old.The proportion of patients aged 61-70 is the highest,the proportion of patients aged 71-80 is the lowest,and the proportion of patients engaged in mental work is relatively high.The average BMI was 22.68±2.53,and the proportion of patients with normal BMI was the highest.The proportion of patients whose first onset season is autumn is the highest,and the proportion of patients whose first onset season is summer is the lowest.The average number of recurrence was 2.81±1.44.The proportion of patients in the period of cold is the highest,and the proportion of patients in the period of hot is the lowest.The proportion of patients with postprandial pain was the highest;The proportion of patients with flatulence pain was the highest;The proportion of patients with pre meal distention was the highest;The patients with acid regurgitation and noise accounted for 66.7%;Nausea accounted for 41.3%;Patients with belching accounted for 67.0%.The proportion of patients whose pain changes with emotion is relatively high;The order of TCM syndromes from high to low was spleen stomach weakness>stomach collateral congestion>liver stomach qi stagnation>spleen stomach damp heat>liver stomach stagnant heat>stomach yin deficiency.The order of the location of internal neoplasia from high to low was antrum>antrum>body>antrum junction.The distribution of TCM syndromes in different parts of the tumor is similar to that in the whole tumor.On the other hand,this study evaluated the related cytological indexes of Shenbai granules.The experimental results show that Shenbai granule has no obvious cytotoxicity on gastric mucosal cells,which indicates that it has good safety and low safety risk in clinical application.In addition,the results of PCR showed that Shenbai granule could reduce the expression of iNOS and COX-2 in inflammatory gastric mucosal cells,which had a certain anti-inflammatory effect. |
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