| Cornus officinalis Sieb.et Zucc.Is a kind of traditional Chinese medicine,which is also a dual-purpose plant.Its pulp contains a large number of phenols,iridoids and other chemical components,which has significant activities of regulating immunity,hypoglycemia,antibacterial and antioxidant,and has a wide range of clinical applications.According to the Chinese Pharmacopoeia,"Cornus is obtained by removing the core of dried Cornus".The core is treated as waste in the process of processing,but at present,the annual output of Cornus flesh is only about half of the market demand.Therefore,in this paper,ultra assisted aqueous two-phase extraction(UAATP)was used to optimize the extraction of polyphenols from the pulp and core of Cornus officinalis,and the differences in chemical composition,bioavailability and antioxidant activity were systematically compared to explore the development value of Cornus The main conclusions are as follows:1.Optimization of uaatp process for polyphenols in the pulp and core of Cornus officinalis:in the aqueous two-phase system formed by 40%ethanol and 19%ammonium sulfate solution,the optimal uaatp process for polyphenols in the pulp of Cornus officinalis was optimized by single factor and Box-Behnken response surface method:ultrasonic power 360 W,particle size 160 mesh,material liquid ratio 55:1(mL:g),under which the total polyphenol extraction was 61.29+1.20 mg GAE/g DW;The optimal extraction process of polyphenols from fruit core was as follows:ultrasonic power 420W,particle size 160 mesh,material liquid ratio 45:1(mL:g).Under this condition,the total polyphenol extraction amount was 372.40±3.25 mg GAE/g DW.At the same time,under the same extraction conditions,the content of total flavonoid content(TFC)and total tannin content(TTC)obtained from the extraction of pulp and core of Cornus officinalis by uaatp was significantly higher than that of water extraction,which led to the conclusion that the extraction efficiency of UAATP was higher.Compared with the results of electron microscope of the residue extracted by UAATP from the pulp and core of Cornus officinalis,under the same particle size,the original pulp and core showed that the tissue was seriously damaged,and the degree of damage to the cells was higher than that of water extraction.At the same time,the content of mono glycoside in the pulp and core extracted by UAATP was significantly higher than that extracted by water,but the chemical composition in the core of Cornus officinalis was far more complex than that extracted by water.At the same time,HPLC fingerprint comparison showed that the chemical components in the core of Cornus officinalis were far more complex than the pulp.2.The bioavailability of polyphenols in the pulp and core of Co s was analyzed by simulated digestion in vitro.The results of UAATP extract from Cornus pulp showed that TPC was the highest in gastric digestion(20.72±0.78 mg GAE/g DW),TFC was the highest in gastric digestion(15.23±0.42 mg RE/g DW);After digestion,TPC was released the highest in oral digestion(243.49±0.54 mg GAE/g DW),while TFC was released the highest in gastric digestion(118.45±0.49 mg RE/g DW).The antioxidant activity of phenols in the digested solution extracted by UAATP from the pulp and core of Comus officinalis was higher than that extracted by water,and was positively correlated with the TPC content.The results of HPLC analysis showed that the overall polyphenol transmittance of Comus officinalis was higher than that of its pulp.According to the Chinese Pharmacopoeia,the content of monoglycoside and loganin was mainly used to evaluate the efficacy of Cornus officinalis,The transmittance of monetin and loganin in the pulp of Cornus officinalis is higher than that in the core of Comus officinalis,while the content of phenolics in the core of Cornus officinalis is much higher than that in the pulp,Therefore,the core of Cornus officinalis can be used for the development of phenolic substances. |
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