Differential Expression Of Circular RNA In The Plasma Of Elderly Patients With Heart Failure

Research objective :Heart failure is one of the most common cardiac diseases in clinic,which is a serious complication and final stage of various heart diseases.Heart failure has become one of the key reasons for repeated hospitalization and death in contemporary elderly people over 60 years old.One of the reasons for this severe phenomenon is the lack of early diagnosis biomarkers for targeted treatment and prognostic evaluation.Circular RNA（circ RNAs）is a kind of endogenous RNA formed by covalent bond connection.Its stable structure,conservative evolution,high expression and specificity make it a potential biomarker.In recent years,a number of research results suggest that circular RNA is involved in the pathophysiological mechanism of cardiac remodeling in heart failure.However,there are few studies on the expression profile of circular RNA in elderly heart failure at this stage,the expression level and mechanism of circular RNA in elderly heart failure are not clear,and the application value of circular RNA in all aspects in elderly heart failure needs further exploration.Therefore,this study will use circular RNA chip technology to determine the expression profile of circular RNA in the plasma of elderly patients with heart failure and normal elderly,explore its differential expression,conduct bioinformatics analysis,and predict the micro RNA（mi RNA）that may bind to the differentially expressed circular RNA,to explore the role of plasma circular RNA in the occurrence and development of senile heart failure.Research methods :In this study,4 elderly patients（over 60 years old）hospitalized in Provincial Hospital Affiliated to Anhui Medical University from October 2020 to October 2021 were selected as the patient group,and 4 healthy elderly people in the same period were selected as the control group.High throughput gene chip technology was used to detect the expression profile of circular RNA in the plasma of the two groups.Taking the fold change > 1.5 times and the P value of independent sample t-test< 0.05 as the cut-off point,the differentially expressed circular RNA molecules between the two groups were screened.Then bioinformatics technology（Target Scan and mi Randa database）was used to annotate the top 10 circular RNA with up-regulated and down-regulated expression and and predict micro RNAs downstream to verify that it contains micro RNAs that plays an important role in heart failure.Research results :According to the detection results of high-throughput gene chip technology,compared with the control group,there were 190 differentially expressed circular RNA in the plasma of patient group（the fold-change > 1.5,P < 0.05）,of which116 were up-regulated and 74 were down-regulated.There may be binding sites between the screened circular RNA with significant differential expression : hsa＿circ RNA＿102228、hsa＿circ RNA＿102227、 hsa＿circ RNA＿102225、hsa＿circ RNA＿027610、hsa＿circ RNA＿006240、hsa＿circ RNA＿103327 and some micro RNAs involved in the pathological process of ventricular remodeling in heart failure : mi R-764,mi R-410-3p,mi R-483-3p,mi R-148b-3p,mi R-204-5,mi R-17-3p and mi R-134-5p.They may participate in the pathological process of heart failure by adsorbing some micro RNAs involved in the pathological process of heart failure.Research conclusions :The expression of circular RNA in the plasma of elderly patients with heart failure and normal elderly was statistically significant.The differentially expressed circular RNA was screened in this study: hsa＿circ RNA＿102228、hsa＿circ RNA＿102227、 hsa＿circ RNA＿102225、hsa＿circ RNA＿027610、hsa＿circ RNA＿006240、hsa＿circ RNA＿103327.They may participate in the occurrence and development of heart failure by acting as a sponge of micro RNAs partially involved in the pathological process of heart failure.