A Study Of Inflammatory Metabolites And Metabolic Pathways In Rhegmatogenous Retinal Detachment Associated With Choroidal Detachment

[Purpose] To identify the unique metabolites and metabolic pathways involved in the pathogenesis of rhegmatogenous retinal detachment(RRD)associated with choroidal detachment(RRDCD),and provide new ideas for clinical diagnosis and treatment.[Method] Using ultra-high-performance liquid chromatography coupled with the quadrupole time-of-flight mass spectrometry method,metabolites in the vitreous fluid of RRDCD and RRD patients were analyzed.The vitreous fluid of idiopathic epimacular membrane(IEMM)patients was analyzed and used as a control group.A total of 42 samples were analyzed to compare the significance of different metabolites between these three groups.[Results] We identified 106 different metabolites between the RRDCD and RRD groups,135 different metabolites between the RRDCD and IEMM groups,and 105 different metabolites between the RRD and IEMM groups(VIP>1,P value<0.05).Compared with the IEMM and RRD group,38 metabolites were expressed differently in the RRDCD group,and 11 metabolic pathways have significantly interfered.Mainly differences in the metabolism of medium-and long-chain fatty acids,sphingolipid metabolism and endocannabinoid signaling pathways.The indicates that the intraocular immune regulation may be disturbed.[Conclusions] Our findings suggest that medium-and long-chain fatty acids metabolism disorders in RRDCD patients may lead to mitochondrial dysfunction,which intensifies reactive oxygen species’ accumulation.Abnormal sphingolipid metabolism may also lead to the development of inflammation in the eye.Previous research has shown that the destruction of the blood-eye barrier may change the microenvironment of immunosuppression in the eye,and immune regulation may play an essential role in the pathogenesis of RRDCD.The endocannabinoid signaling pathway is disturbed or is related to the clinical manifestations of low intraocular pressure in RRDCD patients.Inflammation has a role in RRDCD.Our study suggests that abnormal medium-and long-chain fatty acids metabolism may be an important pro-inflammatory factor.