The Effect Of Jiaoqi Powder On The Quality Of Ulcer Healing In Rats With Gastric Ulcer

ObjectiveThis study is to investigate the effect of jiaoqi powder on quality of ulcer healing through establishing gastric ulcer rat model.To explore the role of Jiaoqi powder in promoting mucosal repair and improving the quality of ulcer healing and its mechanism,so as to provide a reliable modern medical basis for better excavation and inheritance of traditional Chinese medicine academic experience.MethodsTo establish classical gastric ulcer model induced by glacial acetic acid.The rats were divided into 6 groups:sham operation group,model group,Jiaoqi powder group,Omeprazole group,Yunnan Baiyao group,Omeprazole add Jiaoqi powder group.12 rats in each group.The sham operation group and model group were treated with physiological saline solution(10ml/kg~2d);while the Jiaoqi powder group was given intragastrically by a solution of Jiaoqi poeder(540mg/kg ~2 d);the Omeprazole group was given intragastrically by a solution of omeprazole(1.8mg/kg ~2 d);the Yunnan Baiyao group was given intragastrically by a solution of Yunnan Baiyao(90mg/kg~2d);the Omeprazole add Jiaoqi powder group was administrate a solution of omeprazole(1.8mg/kg~2d)add a solution of Jiaoqi poeder(540mg/kg ~2 d)by means of Intragastric administration.Intragastric administration for 10 days.Finally samples were collected,then measuring the ulcer area,calculating the ulcer inhibition rate,observing the gastric mucosal pathological changes.The protein content of IL-2,IL-1βand TNF-α in the serum of ulcerative rats was detected by Elisa.The mRNA expression of IL-2,IL-1β,TNF-α,EGF,VEGF,PGE2 and TFF2 in gastric mucosa was detected by QPRC.ResultsAfter intragastric administration for 10 days,the ulcer area of rats in each administration group was significantly reduced compared with the model group,and the degree of mucosal injury recovery was higher than the model group.The expression of EVGF、PGE2 mRNA in gastric mucosa of Jiaoqi poeder group was significantly higher than the model group(P<0.01);The expression of IL-1βand TNF-α mRNA in the gastric mucosa of rats in Jiaoqi powder group were significantly lower than those in the model group(P<0.01);There was no significant difference in the expression of IL-2,EGF,TFF2 mRNA in gastric mucosa of Jiaqi powder group compared with the model group(P> 0.05).The content of IL-1β and TNF-α in the serum of rats in the jiao qi powder group were significantly lower than the model group(P<0.01,P<0.05).Compared with the model group,the protein content of IL-2 in the serum of Jiaoqi powder group was significantly increased(P<0.01).Compared with Yunnan baiyao group,the protein content of IL-1β and TNF-α in the serum of rats in Jiaoqi powder group were significantly lower(P<0.01,P<0.05).The protein content of IL-2in the serum of Jiaoqi powder group was significantly higher than Yunnan baiyao group groups(P<0.01).ConclusionOn the one hand Jiaoqi powder can improve the expression of VEGF and PGE2 mRNA in mucosal tissue of ulcer rats,then promote the regeneration of injured mucosal blood vessels and improves gastric mucosal blood flow,thereby enhancing the mucosal repair ability;on the other hand,it can also reduce the release of proinflammatory cytokines,increase the expression of anti-inflammatory factors,thereby regulating the inflammation of the mucosal injury balance,to improve the quality of ulcer healing.