Effects Of Anthocyanins From Lycium Ruthenicum Murray On Intestinal Flora And Liver Transcriptome In Obese Mice Induced By High-fat Diet

Objective Obesity is a common metabolic disease closely related to chronic diseases.In recent years,studies have shown that anthocyanins play a certain role in preventing and treating obesity.The purpose of this study was to investigate the effect and possible mechanism of anthocyanins from Lycium ruthenicum Murray on obese mice induced by high-fat diet.Observing the weight loss effect of anthocyanins from Lycium ruthenicum Murray through animal experiments;the possible lipid-lowering mechanism of anthocyanins from Lycium ruthenicum Murray was studied by high-throughput sequencing.We hope to provide some new strategies for the biological effects of anthocyanins from Lycium ruthenicum Murray and obesity treatment.Methods In this study,the anthocyanins from Lycium ruthenicum Murray were prepared by vacuum filtration,concentrated under reduced pressure and freeze-dried to obtain the anthocyanin content.The p H difference method was used to detect the anthocyanin content.In this experiment,C57BL/6J mice were used as the research object.A high-fat diet was used to induce obesity in mice.The mice were given 0.8 % anthocyanins from Lycium ruthenicum Murray solution for 14 weeks of intervention.During the experiment,the body weight,food intake and water consumption of the mice were recorded once a week.After the experiment,the content of T-CHO,TG,HDL-C,LDL-C,ALT and AST in the serum was detected by the colorimetric method of the microplate reader.The liver,fat and small intestine tissue sections were stained by H&E staining to observe the tissue morphology,and further observe liver lipid deposition by oil red O staining.The V3-V4 region of 16 Sr DNA gene in stool samples was determined to study the effect of the anthocyanins from Lycium ruthenicum Murray on microorganisms in mouse stool samples.Brifly,α diversity was used to analyze the abundance and diversity of mouse intestinal flora,β diversity was used to analyze the difference in microbial community composition,LEf Se analysis and LDA score were used to analyze the different flora of each group of mice at different levels.The liver samples were used to study the effect of anthocyanins from Lycium ruthenicum Murray gene transcription levels in obese mice through high-throughput sequencing.Through GO annotation analysis and KEGG pathway enrichment analysis to find out the possible signal pathways of anthocyanins from Lycium ruthenicum Murray on the lipid-lowering effect of high-fat-induced obese mice.Results(1)The total anthocyanin content of anthocyanins from Lycium ruthenicum Murray is 26.0 ± 3.6%,and the main component is Petunidin3-O-[6-O-(4-O-(trans-p-coumaroyl)-α-L-rhamnopyranosyl)-β-D-glucopyranoside]-5-O-[β-Dglucopyranoside].(2)The anthocyanins from Lycium ruthenicum Murray can reduce the body weight of obese mice induced by high-fat diet(P < 0.05),but there were no significant difference in the food intake and water intake of the mice.At the same time,it reduced the levels of T-CHO and LDL-C in the serum(P < 0.05),and reduced lipid deposits in the liver and fat weight in the body(P < 0.05).(3)The anthocyanins from Lycium ruthenicum Murray increased the diversity of intestinal microbes in mice(P < 0.05),changed the community structure of intestinal microbes,and reduced the ratio of Firmicutes/Bacteroidota at the phylum level(P < 0.05).(4)Through transcriptome sequencing,it was found that anthocyanins from Lycium ruthenicum Murray changed the expression of genes in the liver of mice,and 17 signaling pathways related to lipid metabolism and 769 differentially expressed genes were found,such as metabolic and PPAR pathways.Conclusion The anthocyanins from Lycium ruthenicum Murray can reduce the weight of obese mice induced by high-fat diet,reduce fat accumulation in the body,and improve liver and small intestine function.On the one hand,the mechanism of its occurrence may be related to the increase in the diversity of the mouse intestinal flora,especially the decrease in the ratio of Firmicutes/Bacteroidota.On the other hand,it may be related to the difference in gene expression in the metabolic pathway or PPAR signaling pathway.