| Objective:Lewis lung carcinoma model of nude mice were produced by injection of Lewis lung cancer cells,To investigate the effect of sodium tanshinoneⅡA sulfonate combined with cisplatin on the transplanted tumor growth and expressions of EZH2,VEGF and CD31,further explore the mechanism of angiongenesis.Methods:Lewis lung carcinoma model of BALB/c-nu were produced in nude mice.Then the 36 nude mice were randomized into 4 groups:Model group,STS group,DDP group,STS+DDP group.Model group（NS,0.2 m L,qd）,STS group(15 mg·kg-1,0.2 m L,qd),DDP group(2 mg·kg-1,0.2 m L,qod),STS+DDP group（doses as above,0.2 m L,STS,qd,DDP,qod）.Different treatments were served for 28 days,After administration killed nude mice.We observed the weight and volume of transplanted tumors,calculated inhibitory rate,then plotted the tumor growth curve.Real-time quantitative PCR and Western Blot were used to detect the expression levels of EZH2 and VEGF in tumor tissues.Immune histochemical method to evaluate CD31 labeled MVD expression level.Results:Compared with model group,STS group,DDP group,STS+DDP group tumors had lower weight（P<0.05）,volume decreased obviously（P<0.01）,especially in STS+DDP group（P<0.01）.The anti-tumor rate of STS+DDP group（89.74%）was obviously higher than that of STS group（26.92%）and DDP group（60.26%）,tumor growth were restrained.Compared with Model group,the expression levels of EZH2,VEGF,and CD31 in STS group,DDP group,STS+DDP group were significantly decreased（P<0.05）,and the expression levels in STS+DDP group were even lower（P<0.05）.Conclusion:1 Both sodium tanshinoneⅡA sulfonat and cisplatin could inhibit the growth and microangiogenesis of Lewis lung carcinoma transplanted tumor in nude mice,and the combination of sodium tanshinoneⅡA sulfonat and cisplatin had A stronger inhibitory effect.2Sodium tanshinoneⅡA sulfonat combined with cisplatin can significantly inhibit tumor angiogenesis,which may be due to the inhibition of the expression of EZH2,VEGF and CD31. |
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