| Objective: 1.To explore the protective effect of tanshinone iia sodium sulfonate on vascular endothelial cells in rats with severe acute pancreatitis.2.Based on the experimental animal model of rats,to explore the tanshinone iia sodium sulfonate from the perspective of apoptosis of lung tissue Possible mechanism of action.Methods:1.72 clean male SD rats(body weight 220g-270g)were randomly divided into three groups: sham operation group,SAP group,tanshinone IIA sodium sulfonate treatment group(it’s called tanshinone treatment group for short),The rats in the SAP group and tanshinone treatment group were retrogradely injected with 5% sodium taurocholate(1ml/kg)retrogradely into the pancreaticobiliary duct to establish a severe acute pancreatitis with acute lung injury model.The sham-operated group rats only had an open abdomen,turned the duodenum and lightly touched the pancreas.Rats in the tanshinone treatment group were injected intraperitoneally with sodium tanshinone ⅡA sulfonate(20 mg / kg)immediately after successful modeling,and the sham operation group and SAP group were intraperitoneally injected with 0.9% normal saline(NS)after successful modeling.Rats were sampled at 3h,6h and 12 h after modeling.2.Observe the pathological changes of pancreas and lung tissue under an optical microscope.3.Enzyme-linked immunosorbent assay(ELISA)to determine serum v WF,s EPCR,TNF-α levels.4.Tunel method to detect lung tissue endothelial cells apoptosis in situ.5.SP immunohistochemistry method was used to detect the expression of Bax and Bcl-2 protein in lung tissue.6.Lung tissue(w / d)ratio to determine the water content of lung tissue.Results:1 The pathological damage of pancreas tissue in the model group continued to increase with the prolongation of the modeling time,and gradually changed from the original mild edema to scattered bleeding,inflammatory cell infiltration,hemorrhagic necrosis,and bloody ascites.Saponified spots were visible to the naked eye around the pancreatic tissue.Formation,the amount of ascites gradually increased with the prolongation of the modeling time.The lung tissue showed different degrees of inflammatory infiltration at various time phases,and the interstitial and alveolar hemorrhage,edema,and atelectasis appeared in different degrees.At the time point,the pathological scores of pancreas and lung in rats were lower than those in the corresponding period of the model group(p <0.05).2 Serum amylase levels in the model group increased with prolonged modeling time.The tanshinone treatment group was lower than the model group at each time point(P <0.05),but both were higher than the sham operation group(P <0.05)The serum level of TNF-α in the model group began to rise rapidly after modeling,reached a peak at 3h,and slowly decreased at 6h and 12 h.Both were higher than the sham operation group(P <0.05).The model group’s serum v WF level was higher than the sham operation group(P <0.05),reached a peak at 6h,and slowly declined at 12 h,and the tanshinone treatment group at 6h,12 h was significantly lower than the model group(P <0.05),and the serum v WF levels of the rats in the model group and the tanshinone treatment group were higher than those in the sham operation group at the same time point.The s EPCR in the model group increased rapidly after surgery and followed with the model The prolonged time gradually increased,and the tanshinone treatment group reached a peak at 6 hours after surgery,and slowly decreased at 12 hours,which was significantly lower than the model group(P <0.05),but both groups were higher than the sham operation group at the same time point(P <0.05).3 Lung tissue apoptosis indexA small amount of apoptosis was detected in the lung tissue of the sham-operated rats.The apoptotic index of the lung tissue of the model group was significantly higher than that of the sham-operated group at all time points(P <0.05).The tanshinone treatment group’s lung tissue apoptosis index also increased significantly after surgery,and was significantly higher than the model group at each time point(P <0.05).4 Bax and Bcl-2 protein expression in lung tissueThe lung tissue of the sham operation group showed a small amount of Bax and a certain amount of Bc I-2 protein expression,both in the model group and the tanshinone treatment group were significantly expressed at various time periods after modeling(P <0.05),and the Bax protein expression in the model group reached 6 hours.The peak,and then decreased with time,the tanshinone treatment group was higher than the model group at each time period(P <0.05).There was no significant difference in Bcl-2 protein expression between the model group and the tanshinone treatment group.5 Water content of lung tissueCompared with the sham operation group,the lung water content of the model group showed a gradual increase trend,the ratio of each time point was significantly higher than that of the sham operation group(P <0.05),and the tanshinone treatment group had a significantly lower phase point at 12 h.In the model group(P <0.05),but still higher than the sham operation group(P <0.05).Conclusion:1.Tanshinone ⅡA sodium sulfonate has anti-inflammatory,ischemic and other effects,can inhibit the release of tnf-α,can reduce vascular endothelial cell damage in sap rats,thereby reducing pancreatic and lung tissue damage,and severe acute pancreatitis Rats with lung injury have a therapeutic effect.2.In the process of sodium taurocholate-induced SAP and ALI,cell necrosis caused the release of inflammatory factors.Tanshinone IIA sulfonate promotes apoptosis by up-regulating Bax protein,which can reduce the release of inflammatory factors. |
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