Study On The Effects Of Matrine On Cisplatin’s Inhibition Of HepG2 Hepatoma Cell Transplanted Tumor In Nude Mice And Its Protective Effect On Liver And Kidney

Objective: To investigate the effects of matrine(Ma)on cisplatin’s(DDP)inhibition of Hep G2 hepatoma cell transplanted tumor in nude mice and its protective effect on liver and kidney.Methods: Hep G2 hepatoma cells were injected subcutaneously into the axilla of nude mice to construct an experimental animal model of subcutaneous transplantation tumor in nude mice.35 successfully modeled female nude mice(tumor diameter about 5mm)were randomly divided into 5 groups(7 mice in each group): normal saline(NS)control group,DDP(2mg/kg)group,DDP(2mg/kg)+ Ma(25mg/kg)group,DDP(2mg/kg)+ Ma(50mg/kg)group,DDP(2mg/kg)+ Ma(100mg/kg)group.Nude mice were treated with drugs for 14 days after tumor formation,the tumor was taken out,and the tumor weight was weighed with a micrometer electronic scale,and the tumor inhibition rate was calculated by the formula.Take out the mouse kidney and liver,calculate the kidney index and liver index by formulas.Detection of alanine aminotransferase(ALT),aspartate aminotransferase(AST),cholinesterase(Ch E),urea(UREA),creatinine(CRE),and uric acid(UA)in the serum of nude mice.Results: It was observed that the tumor mass in each medication group was significantly lower than that in the normal saline(NS)control group(P<0.05).The tumor mass in each dose of matrine combined with cisplatin group was compared with that in the DDP(2mg/kg)group.Smaller(P<0.05).DDP(2mg/kg)group,DDP(2mg/kg)+ Ma(25mg/kg)group,DDP(2mg/kg)+ Ma(50mg/kg)group,DDP(2mg/kg)+ Ma(100mg/kg)group The tumor rate was58.3%,76.7%,79.6%,and 82.1%.Compared with the DDP(2mg/kg)group,serum cholinesterase increased significantly in the DDP(2mg/kg)+ Ma(25 mg/kg)group,DDP(2mg/kg)+ Ma(100 mg/kg)group(P <0.05).UREA in the DDP(2mg/kg)group was higher than that in the normal control group(P < 0.05),and UREA in the DDP(2mg/kg)+ Ma(25mg/kg)group was significantly higher than that in the DDP(2mg/kg)group(P<0.05),While UREA in the DDP(2mg/kg)+ Ma(50 mg/kg)group and DDP(2 mg/kg)+ Ma(100mg/kg)group has a trend of decline.The renal index of each dose of matrine combined with cisplatin group was higher than that of DDP(2mg/kg)group(P<0.05).Conclusion:The combination of Ma and DDP can inhibit tumor growth and increase the rate of tumor inhibition;the combination of Ma and DDP can promote the function of the liver to synthesize cholinesterase and enhance the body’s liver damage from chemotherapeutic drugs;when high-dose Ma and DDP are used in combination,it can reduce the effect of DDP Kidney damage reduces the toxic and side effects of DDP on the liver and kidneys and achieves a protective effect.