Effect Of Icaritin On Proliferation And Apoptosis Of Human Ovarian Cancer Cells SLOV3

Objective:Based on the PI3K/AKT signaling pathway,the aims of this study were to observe the proliferation and apoptosis of ovarian cancer SKOV3cells when exposed to different concentrations of Icaritin and to explore the possible molecular mechanisms.To provide scientific research experimental basis and clinical medication ideas for exploring new drug treatments for ovarian cancer.Methods:1.The research object was ovarian cancer SKOV3 cells.The cells were divided into the control group and Icaritin groups(5,10,20μmol·L^-1),and drug was administrated for 48 hours.The cell counting kit-8（CCK-8）assay was used to detect the inhibitory effect of Icaritin on the proliferation of ovarian cancer SKOV3 cells.Proliferation ability of the SKOV3 cells was detected by Ed U assay.Hoechst33342 fluorescence staining was used to observe the apoptotic morphology of SKOV3 cells in each group.The distribution of cell cycle and apoptosis rate of each group were detected by flow cytometry.2.Quantitative Real-time PCR was used to detect m RNA levels of PTEN,PI3K and AKT in each group of cells treated with different concentrations of Icaritin.Protein expression of PTEN,PI3K,p-AKT and AKT were measured by Western Blot.Results:1.The results showed that the cell inhibition rates of Icaritin groups were significantly increased compared with the control group（P<0.05）,and the inhibition effect is concentration-dependent.The rates of Ed U-positive cells of Icaritin groups were significantly decreased（P<0.05）in a concentration-dependent manner.SKOV3 cells in Icaritin groups showed morphological changes of apoptosis.Apoptosis rates of Icaritin groups were significantly increased（P<0.05）,and the intervention effect is concentration-dependent.The proportions of cells in G₀/G₁phase of Icaritin groups were decreased（P<0.05）and the proportions of S phase cells were increased（P<0.05）in a concentration-dependent manner.2.The RT-PCR results showed that the gene and protein expression of PTEN in Icaritin groups were elevated with the increasing dose of Icaritin compared with the control group（P<0.05）.The gene expression of PI3K and AKT in Icaritin groups were downregulated with the increasing dose of Icaritin（P<0.05）.The Western blot results demonstrated that the protein expression of PTEN in Icaritin groups were increased with the increasing dose of Icaritin（P<0.05）.The protein expression of PI3K and p-AKT in Icaritin groups were reduced with the increasing dose of Icaritin（P<0.05）.There was no significant difference in protein level of AKT between Icaritin groups and control group（P>0.05）.Conclusion:1.Icarin may inhibit the proliferation of ovarian cancer SKOV3 cells in vitro and induce cell apoptosis in a concentration-dependent manner.2.Icarin may inhibit the proliferation of ovarian cancer SKOV3 cells and induce cell apoptosis and affect the cell cycle distribution by inhibiting the PI3K/AKT signaling pathway.