Effect And Mechanism Of Didang Decoction On NLRP3 Inflammatory Bodies In Diabetic Cardiomyopathy Mice

Objective: To investigate the mechanism of the disease In this study,the expression levels of reactive oxygen species(ROS),nod like receptor 3(NLRP3),thioredoxin interacting protein(TXNIP),cysteinyl aspartate specific proteinase-1(caspase-1),interleukin-1 β(IL-1 β)in myocardial tissue of diabetic cardiomyopathy mice were observed,and the myocardial pathological changes of mice ineach group were observed,so as to explore the effect and mechanism of Didang decoction on NLRP3 inflammasome in diabetic cardiomyopathy mice The purpose of this study is to reveal the mechanism that Didang decoction can improve the inflammatory response by inhibiting the activation of NLRP3 inflammasome,delay the progress of diabetic cardiomyopathy,give full play to the advantages of Didang decoction in breaking blood and removing blood stasis,andprovide a new treatment idea for diabetic cardiomyopathy.method:Sixty C57 BL / 6J mice were randomly divided into normal group(n=10)and model group(n = 50).The diabetic mice model was established by intraperitoneal injection of high-fat diet combined with streptozotocin(STZ)in the model group Four weeks later,the cardiac function of mice was detected byultrasound imaging platform.If the cardiac function was decreased,the diabeticcardiomyopathy mice model was established successfully.After excluding thenon model mice,40 mice were finally established.According to body weight andblood glucose,the mice in the model group were randomly divided into modelgroup,Didang decoction low(1.5g · kg-1),medium(3G · kg-1),high dosegroup(6g · kg-1)and simvastatin group(0.0015 g · kg-1),with 8 mice in each group.The cardiac function of mice was detected by ultrasound imaging platform.The fasting blood glucose(FBG),triglyceride(TG)and total cholesterol(TC)were detected by automatic biochemical analyzer.The pathological changes of myocardium were observed by HE staining.The levels of NLRP3,TXNIP,caspase-1 and IL-1 β in myocardial tissue were detected by Western blot.The content of ROS in myocardial tissue was detected.result:(1)Biochemical results showed that,compared with the normal controlgroup,the blood glucose of the model group was increased,and the symptomsof "three more and less" appeared,the hair color was dim,rare,the action was slow,the reaction ability was low,and the levels of FBG,TC and TG weresignificantly increased,the difference was statistically significant(P < 0.01);compared with the model group,the levels of FBG,TC and TG in the middle and high-dose Didang decoction groups and simvastatin groups were significantlyincreased The change was more obvious in the middle dose group(P < 0.05).(2)The results of echocardiography showed that,compared with the normal control group,EF and FS values were significantly decreased,the difference wasstatistically significant(P <0.01);compared with the model group,EF and FS were improved in each dose of Didang decoction group and simvastatin group,the difference was statistically significant(P < 0.05);the change in middle doseof Didang decoction group was more obvious(P < 0.05).(3)He staining results showed that: in the normal control group,the myocardial fibers were uniform,thecells were complete,and the nucleus was located in the center of the cells,and the size was uniform;while in the model group,the myocardial fiberswere disorderly arranged,the texture was uneven,the cells were hypertrophic and deformed,the cytoplasm and nucleus were separated,the intercellular spacewas increased,and inflammatory infiltration was seen around.Compared with the model group,the myocardial fibers and cells in the medium dose group,high dose group and simvastatin group were improved,and the arrangement of myocardial cells in the medium dose group and simvastatin group was more orderly,the fibers were complete,and the improvement was obvious.(4)The results of immunofluorescence showed that compared with the normal control group,the expression of ROS in the model group was significantly higher than that in the normal control group(P < 0.05);compared with the model group,theexpression of ROS in each dose of Didang decoction group and simvastatin group was significantly decreased(P < 0.05).(5)Compared with the normal control group,the expressions of NLRP3,TXNIP,caspase-1 and IL-1 β in the model group were significantly increased(P < 0.05).Compared with the model group,the expressions of NLRP3,TXNIP,caspase-1 and IL-1 β in myocardial tissue were significantly decreased in each dose group and simvastatin group,and the difference was statistically significant(P <0.05)Simvastatin group had no significant effect on the above expression(P > 0.05).Conclusion:(1)High fat diet and intraperitoneal injection of STZ can successfully make diabetic cardiomyopathy mouse model.(2)Didang decoction to be able to delay the myocyte apoptosis,reduces the inflammatory reaction,improves the diabetic cardiomyopathy mouse cardiomyopathy inflammation pathological change,thus delays the diabetic cardiomyopathy occurrence and the development.The mechanism may be: a certain dose of resistin decoction can reduce the expression of NLRP3,TXNIP,caspase-1 and IL-1 β,improve the level of oxidative stress by regulating ROS / TXNIP / NLRP3 pathway,mediate the inflammatory lesions of myocardial tissue,protect myocardial cells,and improve cardiac function.