Study On Microbiological Index Evaulation And Test Methodology Of Three Kinds Of Heat-clearing Chinese Medicine Preparations And Five Kinds Of Moisturiing Cosmetics

Objective Analyze and evaluate the true microbial contamination of skin care cosmetics for children and TCM preparations for children with heat-clearing,verify the applicability of standard microbiological test methods to samples,establish microbial limit test methods for various varieties,and provide a basis for the improvement of corresponding test standards,And investigate and analyze the microbial contamination in the product.The feasibility of the microcaloric technology based on the microbial growth heat signal to quickly detect the pollution control bacteria Escherichia coli and Staphylococcus aureus in medicines and cosmetics was discussed.Methods1、 The total number of aerobic bacteria,fungi and yeasts in three children’s heat-clearing Chinese medicinal preparations（69 batches）and five children’s emollient cosmetics were counted and the control bacteria checked by calculating the recovery ratio of each test strain Method to investigate the applicability of the method.2、 Use the established method to check the microbial limit of the sample,and after separating and purifying some of the detected microorganisms,carry out strain identification and risk analysis.3、 Through the enrichment culture of the original powder of Xiaoer Zhenbei powder with different irradiation doses,the relationship between it and the number of microorganisms was analyzed,and the appropriate irradiation amount was explored.4、 Use a microcalorimeter to measure the characteristics of the growth thermograms of Staphylococcus aureus and Escherichia coli.By comparing the similarities of the graphs,set the threshold standard for positive detection and conduct methodological investigations.Results1、 69 batches of children’s heat-clearing traditional Chinese medicine preparations were tested for microbial limit projects.Among them,35 batches of Xiaoer Zhenbei San had microbial contamination.After the isolated contaminated colonies were processed,they were subjected to metagenomic sequencing,and the base sequences obtained were subjected to BLAST analysis.These can colonies may be derived from fertilizer,soil,and irrigation water.The microbial contamination in Xiaoer Zhenbei powder with different radiation doses was tested,and it was found that the total number of microbes in the sample with a radiation dose of 1k Gy was similar to that of the sample without radiation.When the radiation dose was 7k Gy and 10 k Gy,the total number of microbes detected was similar.Significantly reduced and flattened.2、 Five batches of moisturizing milk cosmetics for children were tested by the microbiological testing method in the cosmetic hygiene technical specifications,and it was found that the standard methods in the cosmetic specifications were not completely suitable for all samples.Propose a test method that can ensure the accuracy of the results.The recovery ratios of the established new method for the five test strains are all 0.5-2.The control bacteria in the test group can be detected,but none of the negative control groups are detected.3、 Under a certain range of test conditions,the growth thermograms of Staphylococcus aureus and Escherichia coli are characteristic and stable,and they have been successfully used to simulate contamination in lincomycin lidocaine gel samples and quality control samples.Detection of Staphylococcus aureus.Conclusion Among the three kinds of heat-clearing Chinese medicinal preparations for children,Xiaoer Zhenbei San has quality risks and serious microbial contamination.The contamination comes from raw medicinal powders.The raw medicinal powders are sterilized by ⁶⁰Co-γ irradiation,and the radiation dose is discussed.The relationship between the number of microorganisms.The standard method in the cosmetic hygiene technical specifications is not completely applicable to the microbial limit inspection of five batches of moisturizing cosmetics for children,and the newly established method is accurate and reliable,and can effectively control the quality of samples.A new method for the detection of Staphylococcus aureus and Escherichia coli has been established.The established microcalorimetry method has the advantages of sensitivity,rapidity,accuracy and simplicity.It can be used as a rapid detection and confirmation of Staphylococcus aureus and the large intestine in the microbial limit test.The supplement method of Escherichia coli.