| Objective:To investigate the effect of Ginkgo biloba extract(EGB)on the expression of Nitric Oxide(NO)and peroxynitrite(ONOO~-,3-NT indirect reflection)after Hypoxic-ischemic brain damage(HIBD)in neonatal rats,to observe the therapeutic effect of EGB on HIBD liver damage,and for EGB clinical application to provide a certain theoretical basis.Methods:A total of 90 newborn 7-day-old clean SD rats were randomly divided into three groups:sham operation group,model control group and model treatment group.Thirty rats in each group were killed at 6h,12h,24h,48h and 72h after surgery,To remove the right common carotid artery,No ligation is required.A model of hypoxic ischemic liver damage using Rice method,EGB group,by 60 mg/kg,EGB,injected into the abdominal cavity,sham operation group and control group injected normal saline of equal volume.Five time points were selected to collect blood from abdominal aorta,centrifuged,and the supernatant was placed separately to detect Alanine aminotransferase(ALT)and Aspartate aminotransferase(AST),and the rats were sacrificed to collect the specimens.Take some fresh liver tissue homogenate,Detection of NO,by chemical colorimetric nitrate reductase The rest of the tissue is fixed,embedded in paraffin,sliced,HE staining was used to observe the morphology and structure,Immunohistochemical method was used to detect the expression intensity of induced Nitric Oxide synthase(i NOS)、endothelial Nitric Oxide synthase(e NOS)、3-Nitrotyrosine(3-NT)in liver tissue of each group.centrifuged,and the supernatant was placed separately to detect ALT and AST,and the rats were sacrificed to collect specimens.Results:1.Liver function test results:control group and treatment group of liver function compared with sham operation group showed different degrees of damage,the most serious damage at 24h;treatment group compared with the control group,the level of liver function slightly decreased,the situation was more optimistic than the control group,there was significant difference between the two groups(P<0.05).2.Results of NO content determination:the content of control group and treatment group had a downward trend in the early stage,and gradually increased with the time prolongation,reaching the peak at 24h,but the increase of NO content in control group was more significant than that in treatment group,with statistical significance(P<0.05).3.The results of liver HE staining showed that the structure and arrangement of hepatocytes in sham operation group were completely normal at each time point,and there was almost no pathological abnormality.The arrangement of hepatocytes in NS group is very disordered,and a large number of vacuoles can be seen.Over time,hepatocyte degeneration is deteriorating,inflammatory cells increase,and the lesions are the most serious at 24 h.Hepatocyte degeneration and neutrophil infiltration were improved in EGB groups.4.Immunohistochemical results showed that eNOS,iNOS and 3-NT positive staining were all brownish yellow,and brownish yellow positive staining was observed in all three groups.Compared with the sham operation group,the expression of eNOS in control group reached the peak at 6h(P<0.05),and gradually decreased after 12h.The values of i NOS and 3-NT optical density in control group were higher than those in sham group at five time phases(P<0.05).Compared with treatment group,the optical density of e NOS in treatment group was significantly higher than that in control group(P<0.05).The optical density of iNOS and 3-NT in control group and treatment group began to increase at 6h and gradually decreased after reaching the peak value at 24h,but the decrease was more obvious in treatment group than in control group,with statistical significance(P<0.05).Conclusion:1.NO and ONOO~- are involved in the process of liver damage after HIBD.2.EGB can regulate NO and ONOO~-expression to protect the liver tissue after HIBD. |
PDF Full Text Request