Mechanism Of Circ＿0010029/miR-6743-5p/PRDM2 Axis In Regulating Proliferation,invasion And Migration Of Glioma Cells

Objective:To investigate the correlation between circ＿0010029,mi R-6743-5p and PRDM2 in human glioma cells,and to illustrate that circ＿0010029 affects the expression of mi R-6743-5p through sponge adsorption,thereby regulating the expression of PRDM2 and affecting the proliferation,invasion and migration of human glioma cells.Methods:1.The expression levels of circ＿0010029,mi R-6743-5p and PRDM2 in 3normal brain tissues and 21 glioma tissues were detected by q RT-PCR.2.The expression levels of circ＿0010029,mi R-6743-5p and PRDM2 in human normal glial cells(HEB)and malignant glioma cell lines U87 and U251 were detected by q RT-PCR.3.Human glioma cell lines U87 and U251 were transfected with circ＿0010029overexpressing plasmid.Each group of cell lines was divided into two groups: The first group was empty vector group;The second group was overexpressed circ＿0010029(over-circ).4.The expression level of circ＿0010029 in each group after transfection was detected by q RT-PCR,and cell proliferation,invasion and migration ability were detected by CCK-8 method,MTT method,Transwell chamber invasion,Transwell chamber migration,and Wound healing scratch experiment,respectively.5.The expression levels of mi R-6743-5p and PRDM2 in each group after transfection were detected by q RT-PCR.6.The expression level of PRDM2 protein in each group after transfection was detected by Western blot.7.Dual luciferase reporter gene assay(DLR)was used to detect whether there were binding sites between circ＿0010029 and mi R-6743-5p,and whether there were binding sites between mi R-6743-5p and PRDM2.Results:1.Compared to normal brain tissue,the expression levels of circ＿0010029 and PRDM2 in glioma tissue were down-regulated,and the expression levels of mi R-6743-5p were up-regulated.2.Compared with normal human glioma cells(HEB),the expression levels of circ＿0010029 and PRDM2 in glioma cells U87 and U251 were down-regulated.On the contrary,the expression level of mi R-6743-5p was up-regulated.3.The results of CCK-8 test and MTT test showed that the proliferation ability of glioma cells was significantly reduced after circ＿0010029 was overexpressed.Transwell chamber invasion,Transwell chamber migration and Wound healing scratch experiments showed that the invasion and migration abilities of circ＿0010029overexpression group were decreased.4.After the overexpression of circ＿0010029,the expression level of mi R-6743-5p was down-regulated,while the expression level of PRDM2 was up-regulated.5.Western blot assay: PRDM2 protein expression increased in circ＿0010029overexpression group.6.Dual luciferase reporter gene assay(DLR)showed that there were binding sites between circ＿0010029 and mi R-6743-5p,and there were binding sites between mi R-6743-5p and PRDM2.Conclusions:1.Low expression of circ＿0010029 and PRDM2 in human glioma tissues and glioma cell lines U87 and U251.2.Mi R-6743-5p was highly expressed in human glioma tissues and glioma cell lines U87 and U251.3.Circ＿0010029 inhibited proliferation,invasion and migration of glioma cells through mi R-6743-5p/PRDM2 axis.