In Vitro Study On The Effect Of Different Materials On The Repair Of Early Enamel Caries And The Inhibition Of Re-demineralization

Objective:To observe the effect of different materials on early enamel caries repair and inhibition of re-demineralization at different time points,and the effect of sodium fluoride on the therapeutic effect of resin infiltration.Methods:Collected and screened 130 premolars or third molars that met the inclusion criteria,and established artificial enamel caries models in partially saturated acid-buffered demineralization solution.Five samples before(t0)and after(t1)demineralization were randomly selected for SEM observation,and then the remaining samples were randomly divided into group I(scanning electron microscope group,n=40)and group II(microhardness group,n=80),and then evenly divide the I and II groups into 4 groups(n I=10,n II=20)for remineralization treatment for 6 weeks: group A(infiltration resin group),group B(0.1% sodium fluoride)Group),group C(permeable resin + 0.1% sodium fluoride group),group D(blank control group).After 6 weeks of remineralization treatment,all samples were subjected to re-demineralization treatment for 2 weeks.The samples of group I were treated for 6 weeks(t4)and after demineralization(t5)for scanning electron microscopic observation.The samples of group II were measured for surface microhardness at each time point.Results:Scanning electron microscope observation results: All four treatment methods can fill and repair the surface pores of artificial enamel caries.The surface smoothness of the samples in group C is the best after 6 weeks of treatment.After another 2 weeks of demineralization treatment,the surface of each group of samples had a certain degree of demineralization,and the surface integrity of group C was the best.Surface microhardness measurement results: The surface microhardness value of each group of samples increased with time,but they could not be restored to the level before demineralization.Two weeks after the demineralization treatment,the microhardness values of the samples in each group were reduced to varying degrees,but the difference between the samples of group C before and after demineralization was not statistically significant.Conclusion: Both infiltration resin and 0.1% sodium fluoride can effectively repair early enamel caries and inhibit demineralization,and the combination of penetrating resin and 0.1% sodium fluoride has the best effect.