Correlation Study Of Lymphocyte Subsets In Parkinson’s Disease

Objectives:To investigate the correlation between lymphocyte subsets and Parkinson’s disease(PD)and its clinical significance by detecting the distribution of lymphocyte subsets in peripheral blood of patients with Parkinson’s disease(PD).Methods:From December 2017 to December 2018,the selection of 31 patients with PD(N1=31)who were diagnosed in the Department of Neurology,the third affiliated Hospital of Suzhou University,and 15 healthy controls(N2=15)matched with the sex and age of the patients.Venous blood was collected with informed consent,Ues the automatic blood analyzer to detecte percentage of CD3+,CD4+,CD8+,NK,CD 19+,CD8+CD28+,CD8+CD28-cells and the ratio of CD4+/CD8+in the blood of patients and healthy controls.The severity of motor symptoms was evaluated by UPDRS Ⅲ(0 ≤ 10,11 ≤ 20,>20),and the severity of the disease was evaluated by Hoehn-Yahr classification(0 ≤ 2,2.5≤ 3,).The cognitive function of the patients was evaluated with MoCA scale(less than 26 points,≥26 points),and the anxiety and depression of the patients were evaluated with Hamilton anxiety scale(HAMA)(less than 8 points,greater than or equal to 8 points).Results:1.Compared with the control group,the percentage of CD3+,CD4+cells and CD4+/CD8+in peripheral blood of patients with Parkinson’s disease decreased(P<0 05),while CD8+,NK,CD8+CD28+cells increased in patients with PD(P<0 05).There was no significant difference in the proportion of CD19+,,CD8+CD28-between the two groups.2.CD3+T lymphocytes in PD group decreased with the increase of age.(P<0 05).3.In PD group,the number of NK cells in female was lower than that in male,while the number of CD8+CD28+ cells was higher than that in male(P<0.05).4.CD4+T lymphocytes decreased with the increase of UPDRS III score,and the higher the score,the more significant the decrease(P<0.05).5.There was no significant difference in the distribution of lymphocyte subsets among PD patients with different Hoehn-Yahr grades(P>0.05).6.Compared with patients with normal cognition,the proportion of CD8+T lymphocytes in peripheral blood of PD patients with cognitive impairment was significantly lower than that of patients with normal cognition.(P<0.05).7.Compared with PD patients without anxiety,CD8+T lymphocytes in patients with anxiety decreased significantly(P<0.05).8.PD patients with low education level and CD8+T lymphocytes were more likely to develop cognitive impairment(P<0.05).Conclusion:The percentage of CD3+,CD4+cells and CD4+/CD8+in peripheral blood of patients with Parkinson’s disease were significantly decreased(P<0.05),and the proportion of CD8+,CD8+CD28+,NK cells was increased(P<0.05,).There was no significant change in the proportion of CD 19+and CD8+CD28-cells.CD8+T lymphocytes decreased significantly in patients with cognitive impairment and anxiety.