| Objective By observing the effects of Berchemia lineata(L.)DC on the expression of TLRs/MyD88/NF-κB signaling pathways and related inflammatory factors in SOI model rats,to explore the mechanism of Berchemia lineata(L.)DC in the treatment of salpingitis,provide experimental basis for future clinical development and use of such drugs to treat salpingitis.Methods 90 non-pregnant female SPF SD rats were adaptively fed for one week,The random number table method was divided into a sham-operated group and a modeling group.The modeling group was further divided into model group,Jingangteng group,levofloxacin group,Berchemia lineata(L.)DC alcohol extract low,medium,and high-dose groups.A mixture of Staphylococcus aureus,Escherichia coli and Streptococcus haemolyticus B was injected into the bilateral fallopian tubes of rats to establish a model of tubal inflammation.Three rats in each of the sham-operated and modeled groups were randomly selected on days 10 and 20 after modeling,and the morphology of the fallopian tubes and changes in HE sections were observed to confirm the success of modeling.After successful modeling,the rats were divided into 10 rats in each group and given the corresponding drug solution for gavage,which was taken after 30 days of continuous gavage.The tubal alterations were observed by anatomical observation as well as HE staining microscopy.The secretory levels of IL-1β,IL-6,IL-10,TNF-αand ICAM-1 in the serum of each group were detected by ELISA,and the protein expression levels of TLR2,TLR4,MyD88,NF-κB and ICAM-1 in the tubal tissues of each group were detected by Western Blot.The gene expression levels of TLR2,TLR4,MyD88,NF-κB,IL-1β and IL-6 in the fallopian tube tissues of each group were detected by q PCR.Results.1.Modeling results: After anatomical observation and HE staining microscope section observation,the model rats’ fallopian tubes have uneven thickness,asymmetrical shape,and poor tube wall elasticity.Under the microscope,a large number of inflammatory cells infiltrated and cell vacuoles appeared in the fallopian tube tissue,confirming the success of this model.2.Anatomical observation: The fallopian tubes of the rats in the sham operation group are uniform in thickness,and the two fallopian tubes are symmetrical,soft and elastic to the touch;in the model group,the fallopian tubes are uneven in thickness,with asymmetrical shape,poor elasticity,and adhesion to the surrounding tissues.The rest of the fallopian tube tissues of each administration group were improved to varying degrees in comparison with the above conditions.Among them,Berchemia lineata(L.)DC alcohol extract high-dose group and the levofloxacin group improved the best.Observation under HE staining microscope: The fallopian tube section of the sham operation group showed that the lumen was unobstructed,the structure of the tube wall was clear,the cilia were abundant,and the epithelial cells were tightly connected and arranged neatly.The fallopian tube slices of rats in the model group showed that the structure of the fallopian tube lumen was unclear,the cilia were disorderly and missing,cell vacuoles,necrosis,inflammatory cell infiltration in the muscle layer,and partial lumen obstruction.The rest of the oviduct tissue slices of the other administration groups were improved in comparison with the above aspects.Among them,the erchemia lineata(L.)DC alcohol extract high-dose group,the Jingangteng group and the levofloxacin group were the best.3.The ELISA test results showed that compared with the sham operation group,the serum IL-1β,IL-6,TNF-α,ICAM-1levels of the model group increased,and the serum IL-10 level significantly decreased(P<0.01).Compared with the model group,the secretion levels of IL-1β,IL-6,IL-10,TNF-α,and ICAM-1 in the serum of each administration group were improved to varying degrees.Among them,the Berchemia lineata(L.)DC alcohol extract high-dose group and medium groups Serum IL-1β,IL-6,TNF-α,ICAM-1 levels decreased,and serum IL-10 levels increased the most significantly(P<0.05).4.Western Blot test results showed that compared with the sham operation group,the fallopian tube tissue TLR2,TLR4,MyD88,NF-κB and ICAM-1 of the model group increased(P<0.05).Compared with the model group,the expressions of TLR2,TLR4,MyD88,NF-κB,and ICAM-1 in the fallopian tube tissues of each administration group were improved to varying degrees.The expression of TLR2,TLR4,MyD88,NF-κB and ICAM-1 in the oviduct tissue of rats in the Berchemia lineata(L.)DC alcohol extract high-dose group decreased the most significantly(P<0.01).5.PCR detection results showed that compared with the sham operation group,the expression of TLR2,TLR4,MyD88,NF-κB,IL-1β,and IL-6 mRNA in the model group increased(P<0.05).Compared with the model group,the expressions of TLR2,TLR4,MyD88,NF-κB,IL-1β,and IL-6 mRNA in each administration group decreased.Among them,the expressions of MyD88,NF-κB,IL-1β and IL-6 mRNA in the Berchemia lineata(L.)DC alcohol extract high-dose group and levofloxacin group were the most significantly reduced(P<0.05).Conclusion: Berchemia lineata(L.)DC can significantly improve the adhesion between the fallopian tube and surrounding tissues in SOI model rats,promote the absorption of inflammatory lesions in the fallopian tube,improve the tissue damage caused by inflammation in the fallopian tube,reduce the obstruction in the fallopian tube,restore the basic functions of the fallopian tube,and achieve the purpose of dredging the fallopian tube.The mechanism may be related to the fact that Berchemia lineata(L.)DC inhibits the expression of inflammatory factors IL-1β,IL-6,TNF-α,and ICAM-1 in the body,up-regulates the content of IL-10,and blocks the continuous activation of the TLRs/MyD88/NF-κB pathway. |
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