Expression And Correlation Analysis Of Sestrin3、TGF-β1、Smad7 In Endometrioid Endometrial Carcinoma

Objective:To investigate the expression and clinical significance of Sestrin3,transforming growth factor beta 1,TGF-β1 and Smad7 in endometrioid endometrial carcinoma,and to analyze their correlation,so as to provide theoretical basis for further exploration of the pathogenesis and treatment of endometrioid endometrial carcinoma.Methods:Tissue specimens and clinical data of 93 patients with endometrioid endometrial carcinoma(EEC)confirmed by pathology after radical resection were selected from the Department of Pathology of the affiliated Hospital of North Sichuan Medical College from April 2010 to April 2015.At the same time,30 cases of proliferative phase of endometrium tissues(PE)and 40 cases of endometrial hyperplasia without atypia(EH)and endometrial atypical hyperplasia(EAH)were selected as control group.The expression levels of protein Sestrin3,TGF-β1 and Smad7 in the above tissues were detected by immunohistochemical SP method,and the correlation between their expressions and clinicopathological features was observed.The expression of Sestrin3mRNA in endometrioid endometrial carcinoma was analyzed by GEPIA database,and the relationship between the expression of Sestrin3mRNA and FIGO stage and prognosis of patients with endometrioid endometrial carcinoma was analyzed by GEPIA database.Results:1.The positive rate of protein Sestrin3 expression in PE,EH,EAH and EEC tissues decreased successively,which were 93.3%(28/30),87.5%(35/40),62.5%(25/40)and 39.8%(37/93),respectively.The expression of protein Sestrin3 was not the same among groups(P<0.05).The positive rate of protein Sestrin3 expression in EAH and EEC groups was significantly lower than that in PE and EH groups(P<0.05),and the positive rate of protein Sestrin3 expression in EEC group was lower than that in EAH group(P<0.05),but there was no significant difference in protein Sestrin3 expression between PE group and EH group(P>0.05).The positive rate of protein TGF-β1 expression in PE,EH,EAH and EEC tissues increased successively,and the positive rates were 40.00%(12/30),42.50%(17/40),70.00%(28/40)and 87.10%(81/93),respectively,and the difference was statistically significant(P<0.05).The positive rate of protein Smad7 expression in PE,EH,EAH and EEC tissues also increased in turn,and the difference was statistically significant(P<0.05).The positive rates were 30.00%(9/30),45.00%(18/40),67.50%(27/40)and 79.57%(74/93),respectively.The positive rates of protein TGF-β1 and Smad7 in EAH and EEC groups were significantly higher than those in PE and EH groups(P<0.05),but there was no significant difference between PE group and EH group(P>0.05).And,there was no significant difference in the expression of protein Smad7 between EAH group and EEC group(P>0.05).2.The expression of Sestrin3 mRNA in EEC tissue was lower than that in normal endometrial tissue,but the difference of Sestrin3 mRNA expression in them was not statistically significant(P>0.05).In EEC,the expression of Sestrin3 mRNA decreased from FIGO I to FIGO IV stage,but there was no significant difference in the expression of Sestrin3 mRNA in EEC of different stages.3.There was a negative correlation between the expression of protein Sestrin3 and TGF-β1 in EEC(r=-0.342,P<0.05),and a positive correlation between the expression of protein TGF-β1 and Smad7 in EEC(r=0.282,P<0.05),but there was no significant correlation between the expression of protein Sestrin3 and Smad7 in EEC(r-0.139,P>0.05).4.The expression of protein Sestrin3 in EEC was correlated with histological grade(P<0.05),but not with age,depth of myometrial invasion,FIGO stage and lymph node metastasis(P>0.05).And there was no significant difference in the expression of protein TGF-β1 and Smad7 in EEC tissues among different ages,histological grades,depth of myometrial invasion,lymph node metastasis and different FIGO stages.5.In EEC patients,the prognosis of patients with high expression of Sestrin3 mRNA was better than that of patients with low expression of Sestrin3 mRNA,but there was no significant correlation between the expression of protein Sestrin3,TGF-β1,Smad7 and the survival time of EEC patients.Conclusion:1.The differential expression of protein Sestrin3 in PE,EH,EAH and EEC tissues varies from high to low.It is speculated that the down-regulation of Sestrin3 expression participates in the occurrence and development of EEC.2.Protein TGF-β1 and Smad7 are highly expressed in EAH and EEC,but low in PE and EH.It is speculated that TGF-β1 and Smad7 are involved in the occurrence of EEC,and TGFβ1 also promotes the development of EEC.3.The expression of protein TGF-β1 and Smad7 in EEC is positively correlated,it is speculated that they have a synergistic effect on the occurrence and development of EEC,while the expression of protein Sestrin3 and TGF-β1 in EEC is negatively correlated,it is speculated that down-regulating the expression of protein TGF-β1 is one of the anti-cancer mechanisms of Sestrin3.4.The expression of protein Sestrin3 was related to the histological differentiation of EEC,but not to the patient’s age,the depth of tumor myometrial invasion,FIGO stage and lymph node metastasis,while the expression of protein TGF-β1 and Smad7 was not significantly correlated with patient age,depth of tumor myometrial invasion,histological grade,FIGO stage and lymph node metastasis.5.In EEC patient,the prognosis of patients with high expression of Sestrin3mRNA was better than that of patients with low expression of Sestrin3mRNA,but there was no significant correlation between the expression of protein Sestrin3,TGF-β1,Smad7 and the survival time of EEC patients.