Study Of Plasma Sirt1 And Ac-p53 And Early Prognosis In Patients With Acute Ischemic Stroke

【Aims】 To observe the expression levels of Sirt1 and Ac-p53 in peripheral blood plasma of patients with acute ischemic stroke,and to explore the correlation between the expression levels of Sirt1 and Ac-p53 in peripheral blood plasma of patients with acute ischemic stroke and the early prognosis of patients.【Method】 A total of 80 patients with acute ischemic stroke within 3 days(≤3 days)who were admitted to the Stroke Center of the Hospital from June 2020 to December 2020 were collected,Eighty healthy people with matched age and gender in the same hospital during the same period were selected as the control group.General clinical data including gender,age,smoking,alcohol consumption,hypertension,diabetes,low density lipoprotein,NIHSS score.The case group was divided into major atherosclerosis type and cardiogenic embolism type according to TOAST classification,and was divided into two groups according to complicated brain edema,bleeding transformation,and infection,respectively.According to 90-day m RS score,they were divided into good functional outcome group and bad functional outcome group.Peripheral venous blood plasma of all subjects at admission was collected and cryopreserved,and the expression of Sirt1 and Ac-p53 was detected by enzyme-linked immunosorbent assay(ELISA).Statistical analysis software SPSS23.0 was used for data related statistics and analysis.Statistical analysis software SPSS23.0 was used for data related statistics and analysis.The differential expression of Sirt1 and Ac-p53 among groups and prognostic related factors were compared and analyzed by Mann-Whitney U test in two independent samples with non-parametric tests and Chi-square test.【Results】1.There were no significant differences in age,sex,smoking,drinking,diabetes and low density lipoprotein between case group and control group(P > 0.05).The proportion of hypertension in case group was higher than that in control group(P < 0.01).2.There was no significant correlation between the expression levels of Sirt1 and Ac-p53 in plasma of patients with acute ischemic stroke and gender,age,hypertension,diabetes,smoking,drinking alcohol and low density lipoprotein(P > 0.05).3.The expression levels of Sirt1 and Ac-p53 in plasma of patients in case group were higher than those in control group(P < 0.01).4.There was no significant correlation between the expression levels of Sirt1 and Ac-p53 in plasma and the degree of neurological impairment in patients with acute ischemic stroke(P > 0.05).5.There were no significant differences in expression level of Sirt1 in plasma between cardiogenic embolism type and aortic atherosclerosis type in patients with acute ischemic stroke(P > 0.05),and there was no significant difference in expression level of Ac-p53 in plasma between the two groups(P > 0.05).6.There was no significant correlation between the incidence of infection,hemorrhage transformation and cerebral edema and the expression levels of Sirt1 and Ac-p53 in plasma of patients with acute ischemic stroke(P > 0.05).7.In patients with acute ischemic stroke,the expression level of plasma Sirt1 in the good functional outcome group was higher than that in the bad functional outcome group,the conversion rate of cerebral edema and hemorrhage was lower than that in the bad functional outcome group(P <0.05),and there was no significant correlation between the expression level of Ac-p53 and functional outcome(P > 0.05).8.The expression levels of Sirt1 and Ac-p53 in plasma of patients with acute ischemic stroke were positively correlated(r=0.717,P < 0.01).【Conclusions】1.The early prognosis of patients with acute ischemic stroke was related to the expression level of Sirt1 in peripheral blood;2.There was a positive correlation between the expression level of Sirt1 and Ac-p53 in early peripheral blood of patients with acute ischemic stroke.