Parecoxib Sodium Inhibits Nasopharyngeal Carcinoma Cell Migration And Invasion By Regulating EGFR/PI3K/Akt Axis

Objective: The current study investigated the effects of sodium parecoxib on the migration and invasive properties of nasopharyngeal carcinoma cells.Our findings expose the potential for sodium parecoxib as an adjuvant and analgesic for treatment of nasopharyngeal carcinoma.Methods:1.IC50 values were measured for inhibition of nasopharyngeal carcinoma cell proliferation by sodium parecoxib and used to determine appropriate concentrations for administration.2.Scratch tests and Transwell invasion tests were used to observe the effect of sodium parecoxib treatment on the migration and invasion of nasopharyngeal carcinoma cells.3.Expression of EGFR/PI3K/Akt pathway related proteins and downstream migration and invasion related proteins were measured by immunofluorescence and Western blot following sodium parecoxib treatment.Results:1.IC50 values demonstrate that sodium parecoxib inhibited proliferation of nasopharyngeal carcinoma cells in a dose-dependent manner.Inhibition was pronounced and did not increase further between 24 and 48 h when the concentration of the drug was maintained above 100 μM.2.The results of scratch tests and Transwell invasion tests showed that sodium parecoxib inhibited the migration and invasion of nasopharyngeal carcinoma cells.In addition,the IGF-1-stimulation of migration and invasion was reduced.3.Sodium parecoxib significantly upregulated the expression of E-cadherin protein as detected by immunofluorescence and Western blot analysis.4.Western blots also showed that sodium parecoxib significantly down-regulated the expression of PI3 K and p-AKT.Moreover,the drug reduced the IGF-1-stimulation of PI3 K and p-AKT expression.Conclusion: Sodium parecoxib has an inhibitory effect on the migration and invasion of nasopharyngeal carcinoma cells.The mechanism of action may involve the EGFR/PI3K/Akt signaling pathway.