Hsa-miR-150-5p Can Promote The Proliferation And Migration Of Gastric Cancer Cell Line Sgc7901 And Regulate The Expression Of C-myb

Objective : To investigate the effects of miR-150-5p on the proliferation and migration of gastric cancer cell line SGC7901 and find downstream target gene of miR-150-5p.Methods:1.We used qRT-PCR to detect the expression of miR-150-5p in normal gastric epithelial cell line GES-1 and gastric cancer cell line SGC7901.2.After miR-150-5p mimics and its negative control were transfected into SGC7901 cells,the CCK8 and the Wound Healing experiments were applied to explore the influence of miR-150-5p on the proliferation and migration respectively.3.Using the bioinformatics database and bioinformatics analysis to predict the target genes of miR-150-5p.4.QRT-PCR was used to detecte the influence of miR-150-5p up-regulation on its target genes.5.Using Double Luciferase Reporter gene system to test the relationship between miR-150-5p and c-Myb.Results:1.QRT--PCR revealed that the expression of miR-150-5p was1.267±0.031 in SGC7901 cells,which was higher than that in GES-1cells(P<0.01).2.The CCK8 experiments certified that absorbance of miR-150-5p mimic group was 2.069±0.084,which was significantly higher than that of mimic NC group(P<0.01).3.The Wound Healing experiments showed that the migratory metrics of SGC7901 cells of miR-150-5p mimic group was(50.618±1.202)%,which was higher than that of mimic NC group(33.428±1.218)%(P<0.01).4.Through bioinformatics website(miRDB & Target Scan Human &miRTar Base),GEO2 R analysis and literature reading,we found c-Myb,MAP4K4 and ARL13 B,which may relate to miR-150-5p.5.QRT-PCR indicated that the expression levels of c-Myb,MAP4K4 and ARL13 B in miR-150-5p mimic group were0.754±0.043,0.882±0.045 and 0.922±0.049 respectively.The expression levels of c-Myb,MAP4K4 were lower than those in mimic NC group(P < 0.01,P < 0.05).While the c-Myb level was significantly different.6.The Double Luciferase Reporter gene system showed that after co-transfection with miR-150-5p mimic,the fluorescence value of the mutant c-Myb group was 0.969±0.081 when compared to co-transfection with mimic NC,the fluorescence value of the mutant c-Myb group was significantly higher than that of the wild-type c-Myb group,suggesting that miR-150-5p could bind to the 3’UTR of c-Myb(P<0.01).Conclusions:1、miR-150-5p is highly expressed in gastric cancer cell line SGC7901.2、miR-150-5p could promote the proliferation and migration of SGC7901 cells.3、miR-150-5p could down-regulate the expression of c-Myb in SGC7901 cells.