Association Of MYLIP And ABCA1 Gene Polymorphism With Preeclampsia

Objective:1.To investigate the correlation between Myosin regulatory light chain interacting protein gene（MYLIP gene）and ATP-binding cassette transporter A1（ABCA1 gene）polymorphisms and preeclampsia.2.To analyze the correlation between MYLIP and ABCA1 gene polymorphisms and clinical biochemical indicators of preeclampsia.Method:1.Subjects:Pregnant women who delivered at the Department of the Obstetrics of the First Bethune Hospital of Jilin University from June 2017 to April2020.Included 205 patients with epilepsy example early as cases,diagnostic criteria,see the gestational hypertension disease diagnosis and treatment guidelines（2020）,CPT（PE）can be divided into early-onset preeclampsia group（EOPE group）78cases and late-onset preeclampsia group（LOPE group）127 cases of two subgroups,choose the delivery of 145 cases as the control group proceed case-control study.2.Research methods:DNA was extracted from peripheral blood of pregnant women,and the detection of the MYLIP gene rs3757354 and the ABCA1 gene rs2230806 in peripheral blood of pregnant women was performed using Multiplex PCR for targeted next-generation sequencing technology.The polymorphism of rs3757354 of the MYLIP gene and rs2230806 of the ABCA1 gene were analyzed by the SNPSTATS website and SPSS20.0 statistical software,and the correlation between the polymorphism and disease was discussed.Besides,we also compared the clinical test indexes of pregnant women in the case group and the control group,and analyzed the relationship between the polymorphism of the two gene loci and clinical biochemical indexes,to study whether it is correlated with the injury of the target organs.We compared the results,P<0.05 was statistically significant when comparing the PE group with the control group,and P<0.017 was statistically significant when comparing EOPE and LOPE group with the control group.Result:1.Comparison of general data and clinical data between the case group and its subgroups and the control group1.1 Comparison of general data between case group and subgroup and control group（1）Age（Unit:years）:Patients with EOPE were older than those in the control group,with a median age interval of 31.5（28-36）and a median age interval of 30（27-33）in the control group,and the difference was statistically significant（P<0.017）.（2）The proportion of primiparas and pluriparas in the PE group,EOPE group,and LOPE group was 62.9%,67.9%,and 59.8%,respectively,which was significantly higher than that in the control group 20.7%,and the difference was statistically significant（P<0.017）.（3）Gestational age of delivery:The gestational age of PE patients was significantly earlier than that of control group,and the difference was statistically significant（P<0.017）.1.2 Comparison of clinical data between case group and subgroup and control groupThe body mass index,the ratio of triglyceride to high-density lipoprotein,fasting blood glucose,thyroid-stimulating hormone,serum-free thyroxine,triglyceride,systolic blood pressure,diastolic blood pressure,aspartate aminotransferase,alanine aminotransferase,blood urea nitrogen and serum creatinine in the PE group were significantly higher than those in the control group.The serum-free triiodide adenine,high density lipoprotein,hemoglobin,albumin and platelet were lower than those in the control group,and the differences were statistically significant by univariate analysis（P<0.05）.After binary logistic regression analysis,we found that body mass index（BMI）,fasting blood sugar and testing for thyroid-stimulating hormone and the serum free three iodine gland original glycine,for PE group and its subgroups pathogenesis-related mutual independence influence factors,and serum-free thyroxine,triglycerides,and high-density lipoprotein cholesterol（HDL-C）ratio as independent risk factors for PE and LOPE group.2.Relationship between polymorphisms of MYLIP gene rs3757354 with ABCA1 gene rs2230806 and the risk of preeclampsia2.1 Relationship between MYLIP gene rs3757354 locus with the risk of preeclampsia（1）PE group and control group:（1）the CC and CT,TT genotype distribution in PE and the control group differences a statistically significant（P<0.05）,TT genotype frequency in PE group and the control group were 16.1%,6.9%,the difference was statistically significant（chi-square=6.75,P<0.05）,the genotype may for the PE risk factors of the disease,OR_TT=2.67,95%CI（1.21 5.85）.（2）The T allele frequency of rs3757354 locus of MYLIP gene was 32%in the control group and 40%in the PE group,respectively,and there was significant difference in the frequency distribution of allele between the two groups（χ2=3.94,P<0.05）.（2）EOPE group and control group:（1）the CC and CT,TT genotype in EOPE group and control group in the distribution differences statistically significant（P<0.017）,TT genotype frequency in EOPE group and control group were 19%,6.9%,frequency is significantly higher than the control group,the difference was statistically significant（chi-square=8.59,P<0.017）,OR_TT=3.92,95%CI（1.5210.06）.（2）The T allele frequency of rs3757354 locus of MYLIP gene was 32%in the control group and 44%in the EOPE group,respectively,and there was a statistically significant difference in the frequency distribution of allele between the two groups（χ2=6.108,P<0.017）.（3）LOPE group and control group:（1）There was no statistically significant difference in the distribution of genotypes among CC,CT,and TT groups between LOPE and control group（P>0.017）,but the occurrence frequency of TT genotype in LOPE group was 16%,6.9%higher than that in control group,OR_TT=2.15,95%CI（0.91-5.07）.（2）The T allele frequency of rs3757354 locus of MYLIP gene was 32%in the control group and 37%in the EOPE group,with no statistical significance（χ2=1.26,P>0.017）.Conclusions:1.In this study we found that the body mass index（BMI）,fasting blood sugar and testing for thyroid-stimulating hormone and the serum-free three iodine gland original glycine,for PE group and its subgroups pathogenesis-related factors affecting mutual independence,serum free thyroxine,triglycerides and high-density lipoprotein cholesterol（HDL-C）ratio as independent risk factors for PE and LOPE group.2.Polymorphism of rs3757354 locus of the MYLIP gene may be associated with the incidence of preeclampsia,in which TT genotype and T allele may be risk factors for preeclampsia.Patients with TT genotype with preeclampsia have earlier gestational age.3.The rs2230806 polymorphism of the ABCA1 gene may have nothing to do with the incidence of preeclampsia,but this locus may be related to liver function impairment in patients with preeclampsia,and liver function impairment in patients with preeclampsia carrying TT genotype is more serious.