| ObjectiveTo explore the osteogenic effect of hyaluronic acid mixed Bio-Oss bone powder in the preservation of tooth extraction site,so as to provide a basis for the feasibility of the preservation of materials at tooth extraction site of stomatology.Methods32 3-month-old New Zealand white rabbits were randomly divided into four groups,8 in each group.According to the different filling materials in the tooth extraction fossa,they were successively divided into blank control group(group A),hyaluronic acid+Bio-Oss bone powder complex(group B),hyaluronic acid group(group C),and Bio-Oss powder complex group(group D).The treatment of tooth extraction surgery was:intramuscular injection of atropine sulfate,intravenous injection of pentobarbital sodium,attica and epinephrine were successively used for anesthesia.After the anesthesia was stable,minimally invasive tooth extraction forceps were used for complete extraction of the left central incisor of the maxilla.After the width and height of alveolar bone were measured,corresponding materials were implanted in the experimental group,while the blank control group was not treated.Postoperative management:after 3-4 hours,animals wake up,buprenorphine hydrochloride for analgesia.At the end of the 4w and the end of the 12w after the operation,the bone samples of the maxillary anterior teeth were prepared.Bone mineral density,new osteogenic area,height of new alveolar bone,bone resorption and bone mineralization and deposition rate were measured,and morphological and histological changes were observed.Results1.General condition of each group of animals.There were no obvious abnormalities in the gross observation before surgery.There were no signs of infection in the oral cavity or tooth wound.2.Postoperative bone density and new osteogenic area of each group.The bone mineral density and new osteogenic area of the animals at the end of the 4w and 12w were lower than that of the animals at the end of the 4w,and the bone mineral density and new osteogenic area of the animals in group B were significantly lower than that in group A,group C and group D.The height of new alveolar bone in group C and group D was also significantly lower than that in group A.3.Postoperative bone resorption in bone graft area of each group.At the end of 12w after surgery,osteophyte bone resorption of animals was lower than that of group 4w.Compared with group B,osteophyte bone resorption of animals in group B was significantly lower than that in group A,group C and group D,and the level of osteophyte bone resorption in group C and group D was also significantly lower than that in group A.4.Morphological observation results of each group after operation.The alveolar bone of each group at the end of the 4w and the end of the 12w showed different degrees of regeneration.The height of the new alveolar bone in group B was significantly higher than that in group A,group C and group D,and the height of the new alveolar bone in group C and group D was also significantly higher than that in group A.5.Postoperative histological observation results of each group.At the end of 4w after surgery,a large number of hyperplastic bone and bone cells,rich capillaries,osteoid and new fibrous connective tissue appeared in group B.The bone mass of group C and group D was significantly lower than that of group B.The number of bone and bone cells in control group A was significantly lower than that in group B,C and D.At the end of 12w after the operation,bone reconstruction in group B was basically formed.The construction of new bone was still under way in group C and D,and the number of trabeculae was less than that in group B,but more than that in group A.6.Postoperative bone mineralization and deposition rate of each group.At the end of 4w after surgery,the bone mineralized deposition rate of group B,C and D was significantly higher than that of group A,and the bone mineralized deposition rate of group B was also significantly higher than that of group C and D.At the end of 12w of implant material implanted,the bone mineralized deposition rate of each group increased significantly compared with that of the end of 4w,and the bone mineralized deposition rate of group B,C and D was still significantly higher than that of group A,and the bone mineralized deposition rate of group B was also significantly higher than that of group C and D.ConclusionsBio-Oss bone powder can be used as bone replacement material in tooth extraction site preservation to promote the regeneration and repair of alveolar ridge after tooth extraction in New Zealand rabbits,but it is more effective when combined with hyaluronic acid. |
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