| Industrial hemp(Cannabis Sativa),also known as hemp,is an annual herb of Cannabinaceae cannabis genus in cannabis family.It is widely used in textile,material,cosmetic and food industries,and has high economic value.Cannabidiol(CBD)is an important active component of industrial marijuana,which has anti-tumor,antibacterial,anti-inflammatory,antioxidant and other effects,and does not have mental dependence,but there are few reports on the extraction and enrichment technology of CBD from industrial hemp.Therefore,in this study,the extraction process of CBD from industrial hemp was optimized by response surface methodology,and the enrichment process of CBD was optimized by macroporous resin.In addition,the antibacterial and antioxidant activities of the Enrichment products in vitro were evaluated.,which provides a scientific basis for the development and utilization of CBD from industrial hemp.In this experiment,the extraction process of CBD from industrial hemp was optimized.Taking the extraction yield of CBD as the index,on the basis of single factor experiment,the response surface optimization of extraction time,extraction temperature,ethanol concentration and ratio of solid to liquid were studied in detail by Box-Behnken design.According to the developed RSM model,the optimum conditions for obtaining the optimal yield were as follows: extraction78.41 min,extraction temperature of 80℃,absolute ethanol and 25 mL/g solid to liquid ratio.Under these conditions,the extraction yield of CBD was 0.26 ±0.02%,which was close to the predicted value.The results showed that the optimized extraction process is stable,accurate and feasible.On the basis of optimizing the extraction process,the macroporous resins was used to optimize the enrichment of CBD from industrial hemp.Ten kinds of resins were selected to investigate their adsorption characteristics,and the static adsorption experiments showed that the most suitable resin for adsorption was HPD-100,and the adsorption process conformed to Langmuir and Freundlich isothermal model.In order to obtain the best separation parameters,the factors such as pH value of sample solution,sample rate,sample quantity and desorption conditions were further investigated.The optimized adsorption parameters are as follows: CBD initial concentration of 18.6 μg/mL,pH of 3.0,sample loading 2.6 BV,loading rate of 2 BV/h,temperature of 25℃.The optimized desorption parameters are as follows: elution of deionized water,30% ethanol and 90% ethanol for each 5 BV,flow rate of 2 BV/h.CBD content increased4.2 times after enrichment under optimized conditions,and the recovery yield was 83.13%.The results showed that HPD-100 resin revealed a great capablity to enrich cannabinol,which provides a theoretical foundation for the extraction of CBD.In order to evaluate the activity of the enriched products,the antibacterial and antioxidant activities of the enriched products were studied in vitro.The microdilution method showed that the minimum inhibitory concentration(MIC)of the enriched products to Staphylococcus aureus was 5mg/mL,and had no obvious inhibitory effect on Escherichia coli,and the IC50 for DPPH and ABTS was 0.1875 mg/mL and 2.988 mg/mL,respectively.In this study,the reflux extraction process of cannabidiol from industrial hemp flower and leaves was optimized by response surface method for the first time,the process of enrichment of cannabinol by macroporous adsorption resin was optimized,and the preliminary activity of the enriched products was studied.It is proved that the enriched products have strong antioxidant activity and have certain inhibitory effect on Staphylococcus aureus,which provides a theoretical basis for the application of CBD in industrial hemp. |
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