Experimental Study On Camellia Oleifera Cake Extract As Denture Cleaner

Objective:The purpose of this study is to study the inhibitory effect of natural product Camellia oleifera cake on Candida albicans(C.albicans)and its scavenging effect on Candida albicans membrane adhering to polymethyl methacrylate(PMMA)surface,and to evaluate the biosafety of Camellia oleifera cake aqueous solution as denture base cleaner in order to promote the further development and utilization of Camellia oleifera cake.Methods:(1)Camellia oleifera cake was selected as the experimental object,and the drug-carrying plates with different concentrations were made by Agar dilution method,and the bacteriostatic results of the experiment were observed by calculating the colony formation number.Candida albicans solution was added to the special detection board of Bioscreen analyzer,and different concentrations of Camellia oleifera cake were added to each hole.The program was set up to measure the Candida albicans every 1 hour at600 nm wavelength and record continuously for 48 hours.The effect of oil camellia cake on the growth curve of Candida albicans was recorded and analyzed.Using single factor analysis of variance,the test level is α = 0.05;(2)the PMMA specimens containing Candida albicans membrane were soaked in 100,50 mg/m L Filtered soluble cake of Camellia oleifera C.Abel(FSCO),200,100 mg/m L Autoclaved soluble cake of Camellia oleifera C.Abel,Polident and PBS(negative control group).The experiment was divided into soaking for 30 min and soaking overnight(8 h),).The PMMA specimen after immersion was swirled with PBS,and the absorbance of the concussion solution at 600 nm was measured.Using single factor analysis of variance,the test level is α = 0.05;(3)different concentrations of Filtered soluble cake of Camellia oleifera C.Abel and Autoclaved soluble cake of Camellia oleifera C.Abel were added to the96-well plate containing human gingival fibroblasts,and their cytotoxicity was determined by CCK-8 method.At the same time,the PMMA specimens soaked in Camellia oleifera cake were soaked in the cell culture medium,and the human gingival fibroblasts were cultured with this culture medium.The residual toxicity of PMMA immersion solution was determined by CCK-8 method.Using single factor analysis of variance,the test level is α = 0.05.The PMMA specimens were immersed in Filtered soluble cake of Camellia oleifera C.Abel,Autoclaved soluble cake of Camellia oleifera C.Abel,and artificial saliva at intervals and circulated for 7 days to observe whether the soaking solution made the PMMA specimen colored.(4)the inhibitory activity of tea saponin on Candida albicans and its biological safety were studied according to the same method mentioned above.Results:Filtered soluble cake of Camellia oleifera C.Abel,Autoclaved soluble cake of Camellia oleifera C.Abel,and tea saponin aqueous solution could inhibit Candida albicans in a dose-dependent manner.At the same time,it affects the growth curve of Candida albicans.Three kinds of experimental materials soaked in PMMA specimen containing Candida albicans membrane for 30 min,had no obvious cleaning effect,but soaked for 8 h showed inhibitory effect on Candida albicans membrane.The soaking solution of Camellia oleifera cake and tea saponin had cytotoxicity,but there was no obvious cytotoxic residue after soaking in PMMA.During the immersion time of 7 days,the PMMA specimen could be seen to deposit on the surface under stereomicroscope.Conclusion:Camellia oleifera cake and its main component tea saponin can obviously inhibit the planktonic bacteria of Candida albicans.The effect of soaking for a short time(30 minutes)on inhibiting Candida albicans was not good,the soaking time was prolonged,and the effect of anti-inflammatory inhibition of Candida albicans membrane was enhanced.Camellia oleifera cake soaking solution and tea saponin have cytotoxicity,but as soaking solution,there is no toxic residue,the disadvantage is that soaking will change the color of the base.