MiR-190b Promotes Tumor Growth And Metastasis Via Suppressing NLRC3 In Bladder Carcinoma

Purpose: To investigate the potential role of miR-190 b in bladder cancer.Methods: In this study,the clinical samples were obtained and the expression level of miR-190 b was detected by quantitative real-time PCR in bladder cancer tissues and the matched non-cancerous tissues.Then we constructed miR-190 b overexpressing and knocking-down cell lines to explore its effects on the biological behavior of bladder cancer cells.Through Western blotting and bio-extraction,the expression of NLRC3 protein and its relationship with miR-190 b were detected.Subsequently,we examined the co-regulation of NLRC3 and miR-190 b on cell biological behavior through wound healing,Transwell,HUVEC tube formation,and Ed U assays.Finally,to explore the deeper mechanism by which miR-190 b targets NLRC3 to promote bladder tumor growth and metastasis,we performed GSEA analysis and Western blotting.Results: The results showed that miR-190 b was highly expressed in bladder cancer tissues and different cell lines,and promoted the growth and metastasis of bladder tumors.NLRC3 is under-expressed in tumors and can reverse the positive regulatory effects of miR-190 b on cellular biological functions.The effects of NLRC3 and miR-190 b on bladder tumor growth and metastasis were achieved by Wnt/β-catenin and m TOR signaling pathways.Conclusion: We found that miR-190 b inhibits the expression of NLRC3 and promotes the growth and metastasis of bladder tumors through Wnt/β-catenin and m TOR signaling pathways.miR-190 b may be a new target for the treatment of bladder cancer.