| ObjectiveTo investigate the effects of BSP-PTX on the proliferation and apoptosis of human hepatocellular carcinoma Hep G2 cells in vivo and in vitro and its targeting properties.Methods1.BSP-PTX nanoparticles were prepared by using hydrophobically modified Bletillastriata polysaccharides as carriers and paclitaxel as carriers.2.According to the principle of dynamic light scattering,the particle size and potential of nanoparticles were measured by Malvern particle size analyzer,and the morphology of nanoparticles was observed by scanning electron microscopy.3.The inhibitory effects of BSP-PTX and PTX on Hep G2 cell growth were determined by MTT at 1.0ng/ml,2.0ng/ml,4.0ng/ml,8.0ng/ml and 16.0ng/ml.4.Fluorescence staining was used to observe the targeting of drugs into cells by fluorescence microscopy.5.Inhibitory effect of BSP-PTX paclitaxel nanoparticles on Hep G2 cycle and apoptosis by flow cytometry.6.Effect of BSP-PTX on Hep G2-related apoptotic proteins bcl-2,Bax and caspase-3 by Western blot.7.Establishment of Hep G2 liver cancer xenograft model in Kunming mice,which was divided into blank control group,PTX10mg/kg and 15mg/kg groups,BSP-PTX10mg/kg and 15mg/kg groups,to discuss the inhibiting effect of BSPPTX on Hep G2 growth in vivo.Results1.Scanning electron microscopy results show that the nanoparticles(paclitaxel loaded)constructed in this paper have typical spherical properties.The dispersion coefficient of paclitaxel loaded nanoparticles is 0.062,zeta potential is-25.1mv,Z average particle size is 160.5nm,when paclitaxel loaded nanoparticles with 1mg / m L concentration are diluted 10 times and 100 times,the zeta potential of ptxd is-25.4mv and-26.2mv respectively,Z average particle size is 163.9nm and 164.7nm,and the dispersion coefficient is0.124 and 0.237 respectively.2.The fluorescence signal of BSP-PTX group was significantly stronger than that of PTX injection group,indicating that FITC-labeled drugs entered Hep G2 cells,and the amount of BSP-PTX drugs entering cells was significantly higher than that of PTX injection group(P< 0.05).3.The results of MTT assay showed that BSP-PTX and PTX injection significantly inhibited the proliferation of Hep G2 cells,and the degree of inhibition was significantly correlated with drug concentration,but the inhibition rate of BSP-PTX on the growth of Hep G2 cells was significantly higher than that of PTX,P<0.05.4.Compared with PTX control group,paclitaxel loaded nanoparticles significantly reduced the G2/M phase ratio of Hep G2 tumor cells,while tumor cells were significantly blocked at G0/G1;bsp-ptx group played an obvious role in inducing apoptosis,P<0.05.5.Compared with the control group using paclitaxel,the paclitaxel loaded nanoparticle experimental group can significantly inhibit the expression of Bcl-2and up regulate the expression of Bax and Caspase-3 in tumor cells,P<0.05.6.In vivo tumor inhibition test confirmed that the tumor inhibition rates of PTX 10 mg/kg and 15 mg/kg groups,BSP-PTX 10 mg/kg and 15 mg/kg groups were 37.58% and 45.0%,52.35% and 69.80% respectively.At the same dosage,the tumor weight of BSP-PTX group was lower than that of PTX group,P<0.05.In addition,the average body weight of the animals in the administration group was lower than that in the blank control group(P<0.05),but the body weight of the animals in the BSP-PTX 15mg/kg group was higher than that in the PTX group(P<0.05).Conclusions1.The BSP-PTX prepared in this study has good dilution stability,and the paclitaxel-loaded nanoparticles show good solid sphericity.2.BSP-PTX is more targeted than PTX.The drug-loaded nanoparticles synthesized have the ability to target tumor tissue.3.BSP-PTX can significantly induce apoptosis of Hep G2 cells.4.BSP-PTX can affect the expression levels of apoptosis-related proteins Bax and caspase-3 Bcl-2 in Hep G2 cells.5.Baiji polysaccharide-loaded paclitaxel nanoparticles can safely and effectively inhibit the proliferation of human hepatocellular carcinoma Hep G2 cell lines in vitro and in vivo. |
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