Study On The Anti-inflammatory Effect Of Glycosin On Intestinal Mucosa Of Mice With Ulcerative Colitis

ObjectiveTo study and analyze the anti-inflammatory effect of physalin on intestinal mucosa of mice with ulcerative colitis.Methods90 BALB/c mice were selected as the study subjects,and the mice were randomly divided into normal group,model group,sulfasalazine group,phloretin(low dose group),phloretin(medium dose group)and phloretin(high dose group)by random number table method,with 15 mice in each group.Sulfanilamide pyridine group,model group,liu n leather armor(low dose group),qin leather element(middle dose group)and qin leather element)(high dose group were given dextran sodium sulfate(DSS)preparation of mice UC model,the normal group,model group mice were given normal saline lavage treatment,willow nitrogen sulfanilamide pyridine group was given 200 mg/kg SASP lavage treatment,leather armor(low dose group),qin qin leather element(middle dose group)and qin leather element(high dose group)were given 75 mg/kg,150mg/kg and 300 mg/kg lavage treatment.After the treatment,DAI score was used to observe the general condition of mice.Western blot analysis of the expression levels of Cox-2 and INOS in the intestinal tissues of mice induced by EH.TDI score was used to observe intestinal mucosal injury.ICH detected the distribution and expression of Cox-2 and INOS.And explore the effect of EH on the Nrf2 pathway.Resultswillow nitrogen sulfanilamide pyridine group,qin leather armor(low dose group),leather(middle dose group),qin qin leather element(high dose group)mice DAI scores were significantly lower than model group mice(P < 0.05),and qin leather armor(high dose group)mice DAI score significantly lower than other groups(P < 0.05),qin leather armor(middle dose group)mice DAI score is significantly lower than the qin leather element,low dose group(P <0.05);Western blot results showed that the expression levels of cox-2 and INOS in the intestinal tissues of UC mice in the sulfapyridine group,the low-dose group,the medium-dose group and the high-dose group decreased to varying degrees(P<0.05),and showed a dose-dependent effect.TDI scores of mice in the medium dose group and salazoline-sulfapyridine group were significantly lower than those in the model group(P<0.05),and intestinal mucosal inflammation of mice was less.Cox-2 and INO were strongly positive in the model group,while cox-2 and INO were weakly positive in the medium dose of zine and sulfasapyridine groups.Nrf2 protein was highly expressed in the model group,the sulfasapyridine group and the medium dose group of norepinephrine(P<0.05),and Nrf2 protein level in the intestinal tissues of UC mice in the medium dose group of norepinephrine and the sulfasapyridine group was significantly higher than that in the model group(P<0.05).The difference between the two groups was statistically significant.ConclusionPerzine is effective in the treatment of UC mice,which may be related to its role in the regulation of Cox-2,INOS and Nrf2 pathway related proteins.